Person: Forrest, Kerrie L.
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
Forrest
First Name
Kerrie L.
Name
Forrest, K.L.
ORCID ID
0000-0001-6529-86788 results
Search Results
Now showing 1 - 8 of 8
- Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations(Frontiers, 2024) Singh, D.; Ziems, L.; Chettri, M.; Dracatos, P.M.; Forrest, K.L.; Bhavani, S.; Singh, R.P.; Barnes, C.W.; Noroña Zapata, P.J.; Gangwar, O.; Kumar, S.; Bhardwaj, S.; Park, R.F.
Publication - Relocation of Sr48 to chromosome 2D using an alternative mapping population and development of a closely linked marker using diverse molecular technologies(MDPI, 2023) Nsabiyera, V.; Qureshi, N.; Jianbo Li; Randhawa, M.S.; Peng Zhang; Forrest, K.L.; Bansal, U.; Bariana, H.S.
Publication - Identification and characterisation of stripe rust resistance genes Yr66 and Yr67 in wheat cultivar VL gehun 892(MDPI, 2022) Bariana, H.S.; Kant, L.; Qureshi, N.; Forrest, K.L.; Miah, H.; Bansal, U.
Publication - Adult plant stem rust resistance in durum wheat glossy huguenot – Mapping, marker development and validation(Research Square, 2021) Mago, R.; Chunhong Chen; Xiaodi Xia; Whan, A.; Forrest, K.L.; Basnet, B.R.; Perera, G.; Chandramohan, S.; Randhawa, M.S.; Hayden, M.; Bansal, U.; Huerta-Espino, J.; Singh, R.P.; Bariana, H.S.; Lagudah, E.
Publication - High-density mapping of triple rust resistance in barley using DArT-Seq markers(Frontiers, 2019) Dracatos, P.M.; Haghdoust, R.; Singh, R.P.; Huerta-Espino, J.; Barnes, C.W.; Forrest, K.L.; Hayden, M.; Niks, R.; Park, R.F.; Singh, D.The recent availability of an assembled and annotated genome reference sequence for the diploid crop barley (Hordeum vulgare L.) provides new opportunities to study the genetic basis of agronomically important traits such as resistance to stripe [Puccinia striiformis f. sp. hordei (Psh)], leaf [P. hordei (Ph)], and stem [P. graminis f. sp. tritici (Pgt)] rust diseases. The European barley cultivar Pompadour is known to possess high levels of resistance to leaf rust, predominantly due to adult plant resistance (APR) gene Rph20. We developed a barley recombinant inbred line (RIL) population from a cross between Pompadour and the leaf rust and stripe rust susceptible selection Biosaline-19 (B-19), and genotyped this population using DArT-Seq genotyping by sequencing (GBS) markers. In the current study, we produced a high-density linkage map comprising 8,610 (SNP and in silico) markers spanning 5957.6 cM, with the aim of mapping loci for resistance to leaf rust, stem rust, and stripe rust. The RIL population was phenotyped in the field with Psh (Mexico and Ecuador) and Ph (Australia) and in the greenhouse at the seedling stage with Australian Ph and Pgt races, and at Wageningen University with a European variant of Psh race 24 (PshWUR). For Psh, we identified a consistent field QTL on chromosome 2H across all South American field sites and years. Two complementary resistance genes were mapped to chromosomes 1H and 4H at the seedling stage in response to PshWUR, likely to be the loci rpsEm1 and rpsEm2 previously reported from the cultivar Emir from which Pompadour was bred. For leaf rust, we determined that Rph20 in addition to two minor-effect QTL on 1H and 3H were effective at the seedling stage, whilst seedling resistance to stem rust was due to QTL on chromosomes 3H and 7H conferred by Pompadour and B-19, respectively.
Publication - Genes Sr2/Yr30 and Lr34/Yr18/Sr57 interact to confer enhanced adult plant resistance to the three rust diseases of common wheat(2018) Randhawa, M.S.; Singh, R.P.; Lan, C.; Basnet, B.R.; Bhavani, S.; Huerta-Espino, J.; Forrest, K.L.; Hayden, M.
Publication - Interactions among genes Sr2/Yr30, Lr34/Yr18/Sr57 and Lr68 confer enhanced adult plant resistance to rust diseases in common wheat (Triticum aestivum L.) line 'Arula'(Southern Cross Publishing Group, 2018) Randhawa, M.S.; Lan, C.; Basnet, B.R.; Bhavani, S.; Huerta-Espino, J.; Forrest, K.L.; Hayden, M.; Singh, R.P.Common wheat line Arula displays an acceptable level of adult plant resistance (APR) to stripe rust (YR), leaf rust (LR) and stem rust (SR) in Mexico, and to SR (Ug99 races) in Kenya. Present study was conducted to identify genetic loci that confer resistance in Arula and to understand their interactions for rust resistance. A recombinant inbred line (RIL) population (190 lines) developed from the cross of Arula with susceptible parent Apav was phenotyped under artificially created epidemics of the three rusts in 2014, 2015 and 2016 in Mexico, and for stem rust (Ug99) during the off and main seasons of 2015 in Kenya. The RIL population and parents were genotyped with Sr2/Yr30-linked simple sequence repeat (SSR) marker gwm533 and two sequence tagged site (STS) markers (Lr34/Yr18/Sr57-csLV34 and Lr68-csGS) in addition to genotyping using Illumina iSelect 90K SNP array. A genetic map of 2,634 polymorphic markers (2,631 SNPs, 1 SSR and 2 STS markers) was constructed to locate the resistance loci. Composite interval mapping (CIM) was conducted to identify quantitative trait loci (QTL) associated with rust resistance using phenotypic and genotypic data in Windows QTL cartographer version 2.5. Two consistent QTL contributed by Arula were detected on chromosomes 3BS and 7DS, which corresponded to the previously known APR genes Sr2/Yr30 and Lr34/Yr18/Sr57, respectively. Sr2/Yr30 explained 1.1-14.7% and 41.0-61.5% of the phenotypic variation for YR and SR, respectively; whereas Lr34/Yr18/Sr57 accounted for 22.5-78.0%, 40.0-84.3% and 13.8-24.8% of the phenotypic variation for YR, LR and SR, respectively. Arula was also found to carry the positive allele for marker csGS closely linked to gene Lr68 on chromosome 7BL, although this gene was not detected using CIM. Based on presence or absence of identified genes/QTLs, lines were classified into different groups and t-tests were used to compare the mean relative area under disease progress curve (relAUDPC) over years of different gene combinations. RILs carrying both Lr34/Yr18/Sr57 and Lr68 showed significantly higher APR to LR showing the benefit of gene combination Lr34/Yr18/Sr57 + Lr68. Our results show that RILs possessing both Sr2/Yr30 and Lr34/Yr18/Sr57 had significantly enhanced APR to all three rusts in field trials conducted in Mexico and Kenya. Strategic utilization of these two pleiotropic, multi-pathogen resistance genes with other minor genes is recommended to develop durable rust resistant wheat cultivars.
Publication - Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars(National Academy of Sciences, 2013) Cavanagh, C.R.; Shiaoman Chao; Shichen Wang; Huang, B.E.; Stephen, S.; Kiani, S.; Forrest, K.L.; Saintenac, C.; Brown-Guedira, G.; Akhunova, A.; See, D.; Guihua Bai; Pumphrey, M.; Tomar, L.; Wong, D.; Kong, S.; Reynolds, M.P.; Lopes, M.; Bockelman, H.; Talbert, L.E.; Anderson, J.; Dreisigacker, S.; Baenziger, P.S.; Carter, A.; Korzun, V.; Morrell, P.L.; Dubcovsky, J.; Morell, M.K.; Sorrells, M.E.; Hayden, M.; Akhunov, E.Domesticated crops experience strong human-mediated selection aimed at developing high-yielding varieties adapted to local conditions. To detect regions of the wheat genome subject to selection during improvement, we developed a high-throughput array to interrogate 9,000 gene-associated single-nucleotide polymorphisms (SNP) in a worldwide sample of 2,994 accessions of hexaploid wheat including landraces and modern cultivars. Using a SNP-based diversity map we characterized the impact of crop improvement on genomic and geographic patterns of genetic diversity. We found evidence of a small population bottleneck and extensive use of ancestral variation often traceable to founders of cultivars from diverse geographic regions. Analyzing genetic differentiation among populations and the extent of haplotype sharing, we identified allelic variants subjected to selection during improvement. Selective sweeps were found around genes involved in the regulation of flowering time and phenology. An introgression of a wild relative-derived gene conferring resistance to a fungal pathogen was detected by haplotype-based analysis. Comparing selective sweeps identified in different populations, we show that selection likely acts on distinct targets or multiple functionally equivalent alleles in different portions of the geographic range of wheat. The majority of the selected alleles were present at low frequency in local populations, suggesting either weak selection pressure or temporal variation in the targets of directional selection during breeding probably associated with changing agricultural practices or environmental conditions. The developed SNP chip and map of genetic variation provide a resource for advancing wheat breeding and supporting future population genomic and genome-wide association studies in wheat.
Publication