Person: McLean, S.D.
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McLean
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S.D.
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McLean, S.D.
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- Aptitud combinatoria de líneas de maíz tropical con diferente tipo de mazorca(Sociedad Mexicana de Fitogenética, 2001) Vergara Avila, N.; Rodríguez Herrera, S.A; De Leon Castillo, H.; McLean, S.D.; Vasal, S.K.
Publication - Optimization of an in vitro bioassay for wheat diseases(CIMMYT, 2000) Salgado, M.; Pellegrineschi, A.; Mezzalama, M.; McLean, S.D.; Hoisington, D.A.Alternaria triticina, Fusarium graminearum, Pyrenophora tritici-repentis, Blpolaris sorokinisna (Helminthosporium sativum); Pythium sp., and Rhizoctonia sp. were tested on the basis of being a representative group of important fungal pathogens in order to establish a reliable bloassay system to test eventual fungal resistance in transgenic plants. The inoculum was prepared on V-8 agar medium (in petri dishes) for P. tritici-repents and Pythlum sp., and on PDA (potato dextrose agar) medium for the other fungi. The cultures were incubated at room temperature for 7- 10 days in a culture chamber with a constant standard illumination. The suspensions of conidia or micella were prepared on sterile distilled water with 1 few drops of tween 20 and scrapings of the fungi culture. The inoculum was homogenized by vorlexing the suspension for a few seconds. The concentration used for the conidia solution was adjusted to 106 conidia/ml. Fresh leaf samples from adult plants (heading stage) were sterilized and then dipped In the inoculum suspension. The inoculated leaves were then transferred to water agar medium (1% agar), in 8-well rectangular multi-dishes, at room temperature. The level of resistance of the material to these pathogens was evaluated 4-7 days after inoculation. We believe that the routine use of this protocol (fast, reliable, and inexpensive) could allow the easy identification of resistant/tolerant plants to these diseases. In addition, because of the simplicity of this test, it can be useful for early screening of a large number of individuals, as required for transgenic plant screenings.
Publication - Laboratory protocols: CIMMYT applied genetic engineering laboratory(CIMMYT, 1999) Bohorova, N.; Fennell, S.; McLean, S.D.; Pellegrineschi, A.; Hoisington, D.A.This manual provides the theoretical and technical basis required for the successful establishment of maize and wheat somatic cell tissue cultures that are suitable for in vitro genetic manipulation through the use of biolistic-based and Agrobacterium-mediated gene transfer systems for maize and wheat. Achieving effective transfer integration, and expression of transgenes using appropriate gene constructs promotes understanding of the process of gene transfer and the nature of transgenic plants. In addition the manual introduces readers to the basics of plant genome analysis and its application to the analysis of both maize and wheat transgenic plants. Also covered in this manual are the isolation, digestion, electrophoresis, and transfer of DNA to blots; the non-radioactive hybridization and detection technology in use at CIMMYT, and the application of polymerase chain reaction (PCR) technology to the analysis of transgenic plants. To complete the coverage of the topic, information on phenotypic screening of transgenic plants in the biosafety greenhouses is also presented.
Publication - CIMMYT Maize Program, strategic plan: 1995 and beyond(KARI, 2004) Murenga, M.G.; Mugo, S.N.; Odhiambo, B.; McLean, S.D.; Taracha, C.The application of biotechnology and its wide range of tools pose new challenges to governments that require biosafety frameworks be in place for the safe and responsible use of the genetically modified organisms and their products. However, there is a need for all stakeholders to be more serious about institutional learning, and change through a balanced approach. The focus should be on the most relevant and available scientific basis for the debate on biotechnology and its tools in addition to other life sciences contributing to the solutions to hunger and poverty in the world over. Today, ‘genetic engineering’ is increasingly being applied in agricultural and horticultural research, health, environmental protection, industry and social sciences. However, ‘fears’ and ‘concerns’ raised about ethical and safety aspects have emerged that call for safe and responsible application of modern biotechnology to ensure avoidance of inadvertent harm to human health, environment and biodiversity. To ensure that these c ncerns are addressed biotechnology should be developed and applied within the framework of biosafety. These frameworks should include putting in place appropriate biotechnology and biosafety legal provisions, regulations and guidelines related to specific use. To this end, the government of Kenya through the National Council for Science and Technology (NCST) developed and published the Guidelines for Biosafety in Biotechnology in 1998. In the same breadth, the institutional Advisory Committee on Biosafety (KACB) developed a draft for KARI’s Guidelines and Regulations for Biosafety in Biotechnology Research in 1994. These biosafety guidelines outline the general biosafety policies and procedures of the Kenya Agricultural Research Institute (KARI) to ensure the safety of research with 1 genetically engineered organisms, that of the products, the personnel, agriculture, environment and biodiversity. The methods for the safe handling of transgenic materials in both the biosafety greenhouse and/or laboratory envir nment are described in the Guidelines for Biosafety in Biotechnology Research. The general applicability of these guidelines on genetic modifications include all recombinant DNA (rDNA) methodologies. Information about handling transgenic plants in biosafety greenhouses in Kenya is however, relatively sparse. Currently, though, there is no single source of practical guidance on managing biosafety greenhouses containing transgenics, nor on the requirements for building or renovating plant growth facilities to make them suitable for containing transgenic plants, the products and associated organisms. KARI in conjunction with International Centre for Maize and Wheat Improvement (CIMMYT), with the support of Syngenta Foundation for Sustainable Agriculture, working on the Insect Resistant Maize for Africa (IRMA) project have developed a biosafety level II greenhouse complex (hereinafter referred to as biosafety greenhouse) at the Kenya Agricultural Research Institute’s Biotechnology Centre. These facilities are new nd operational. The biosafety greenhouse contains three greenhouses, which meet the national biosafety standards based on the National Biosafety Committee (NBC) and the KARI Advisory Committee on Biosafety (KACB) requirements. These biosafety greenhouses at KARI Biotechnology Centre serve as bio-containment facilities that provide highly effective means of isolation and prevention of unintended transmission of genetic material. The disposal of genetic material from maize is prevented through reproductive, spatial and temporal isolation.
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