Show simple item record

Molecular characterization of the Puroindoline a-D1b allele and development of an STS marker in wheat (Triticum aestivum L.)

Author: Feng Chen
Author: Fuyan Zhang
Author: Craig Morris
Author: He Zhonghu
Author: Xianchun Xia
Author: Dangqun Cui
Year: 2010
ISSN: 0733-5210
Abstract: Kernel hardness is mainly controlled by one major genetic locus on the short arm of chromosome 5D in bread wheat. Twelve Chinese and CIMMYT wheat cultivars were characterized for the deletion region of Pina-D1b genotype and developing a novel STS marker for this allele. PCR and SDS-PAGE were used to confirm the Pina-D1b genotype, and then 20 pairs of primers were designed to amplify the fragment including deletion region in Pina-D1b genotype by primer walking strategy. An STS marker Pina-N spanning deletion region in Pina-D1b was developed and sequencing results showed that all of 10 Pina- D1b genotypes uniformly possessed a 15,380 bp deletion in comparison with that of Chinese Spring with wild type. This study provided an alternative method to exam Pina-D1b by molecular marker and will accelerate identification of puroindoline alleles in bread wheat
Language: English
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Region: Global
Pages: 80-82
Journal issue: 1
Journal: Journal of Cereal Science
Journal volume: 52
DOI: 10.1016/j.jcs.2010.03.006
Keywords: kernel hardness
Keywords: Wheat
Keywords: puroindoline

Files in this item


This item appears in the following Collection(s)

  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP
  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

Show simple item record