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A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

Author: Liu, L.
Author: Wang, A.
Author: Appels, R.
Author: Ma, J.
Author: Xia, X.
Author: Lan, P.
Author: He Zhonghu
Author: Bekes, F.
Author: Yan, Y.
Author: Ma, W.
Year: 2009
URI: http://hdl.handle.net/10883/2722
Abstract: High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki.
Language: English
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Region: Global
Pages: 295-301
Journal issue: 2
Journal: Journal of Cereal Science
Journal volume: 50
Keywords: Triticum aestivum L.
Keywords: Quality
Keywords: SDS-PAGE
Keywords: MALDI-TOF-MS
Keywords: HMW-GS


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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