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A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

Author: Liu, L.
Author: Wang, A.
Author: Appels, R.
Author: Ma, J.
Author: Xia, X.
Author: Lan, P.
Author: He Zhonghu
Author: Bekes, F.
Author: Yan, Y.
Author: Ma, W.
Year: 2009
Abstract: High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki.
Language: English
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Type: Article
Region: Global
Pages: 295-301
Journal issue: 2
Journal: Journal of Cereal Science
Journal volume: 50
Keywords: Triticum aestivum L.
Keywords: Quality
Keywords: SDS-PAGE
Keywords: MALDI-TOF-MS
Keywords: HMW-GS

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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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