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Molecular characterization of Pina and Pinb allelic variations in Xinjiang landraces and commercial wheat cultivars

Author: Wang, L.
Author: Li, G.
Author: Xia, X.
Author: He Zhonghu
Author: Mu, P.
Year: 2008
Abstract: Grain hardness plays an important role in determining both milling performance and quality of the end-use products produced from common or bread wheat. The objective of this study was to characterize allelic variations at the Pina and Pinb loci in Xinjiang wheat germplasm for further understanding the mechanisms involved in endosperm texture formation, and the status of grain texture in Chinese bread wheat. A total of 291 wheat cultivars, including 56 landraces, and 95 introduced and 140 locally improved cultivars, grown in Xinjiang, were used for SKCS measurement and molecular characterization. Among the harvested grain samples, 185 (63.6%), 40 (13.7%), and 66 (22.7%) were classified as hard, mixed and soft, respectively. Eight different genotypes for the Pina and Pinb loci were identified, including seven previously reported genotypes, viz., Pina-D1a/Pinb-D1a, Pina-D1a/Pinb-D1b, Pina-D1b/Pinb-D1a, Pina-D1a/Pinb-D1p, Pina-D1a/Pinb-D1q, Pina-D1a/Pinb-D1aa, Pina-D1a/Pinb-D1ab, and a novel Pinb allele, Pinb-D1ac. This new allele, detected in Kashibaipi (local landrace) and Red Star (from Russia) has a double mutation at the 257th (G to A substitution) and 382nd (C to T substitution) nucleotide positions of the coding region. Pina-D1b, Pinb-D1b, and Pinb-D1p were the most common alleles in Xinjiang wheat germplasm, with frequencies of 14.3%, 38.1% and 28.6% in hard textured landraces, 25.5%, 56.9% and 11.8% in hard introduced cultivars, and 24.8%, 47.8% and 26.5% in hard locally improved cultivars, respectively. The restriction enzymes ApaI, SapI, BstXI and SfaNI were used to identify Pinb-D1ab or Pinb-D1ac, Pinb-D1b, Pinb-D1e and Pinb-Dg, respectively, by digesting PCR products of the Pinb gene. The unique grain hardness distribution in Xinjiang bread wheat, as well as the CAPs markers for identification of the Pinb alleles provided useful information for breeding wheat cultivars with optimum grain textures.
Language: English
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Region: Global
Pages: 745-752
Journal issue: 3
Journal: Euphytica
Journal volume: 164
Keywords: Bread wheat
Keywords: Triticum aestivum
Keywords: Grain hardness
Keywords: CAPs marker

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  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP
  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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