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An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat

Author: Mago, R.
Author: Brown-Guedira, G.
Author: Dreisigacker, S.
Author: Breen, J.
Author: Jin, Y.
Author: Singh, R.P.
Author: Appels, R.
Author: Lagudah, E.S.
Author: Ellis, J.
Author: Spielmeyer, W.
Year: 2011
ISSN: 0040-5752
Abstract: The stem rust resistance gene Sr2 has provided broad-spectrum protection against stem rust (Puccinia graminis Pers. f. sp. tritici) since its wide spread deployment in wheat from the 1940s. Because Sr2 confers partial resistance which is difficult to select under field conditions, a DNA marker is desirable that accurately predicts Sr2 in diverse wheat germplasm. Using DNA sequence derived from the vicinity of the Sr2 locus, we developed a cleaved amplified polymorphic sequence (CAPS) marker that is associated with the presence or absence of the gene in 115 of 122 (95%) diverse wheat lines. The marker genotype predicted the absence of the gene in 100% of lines which were considered to lack Sr2. Discrepancies were observed in lines that were predicted to carry Sr2 but failed to show the CAPS marker. Given the high level of accuracy observed, the marker provides breeders with a selection tool for one of the most important disease resistance genes of wheat.
Language: English
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Region: Global
Pages: 735-744
Issue: 4
Volume: 122
DOI: 10.1007/s00122-010-1482-7
Journal: Theoretical and Applied Genetics

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  • Wheat for CIMMYT Staff
    Wheat - breeding, phytopathology, physiology, quality, biotech. This collection publications is only accessible by CIMMYT staff.

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