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Laboratory protocols: CIMMYT applied genetic engineering laboratory

Author: Bohorova, N.
Author: Fennell, S.
Author: McLean, S.
Author: Pellegrineschi, A.
Author: Hoisington, D.
Year: 1999
ISBN: 970-648-041-2
URI: http://hdl.handle.net/10883/628
Abstract: This manual provides the theoretical and technical basis required for the successful establishment of maize and wheat somatic cell tissue cultures that are suitable for in vitro genetic manipulation through the use of biolistic-based and Agrobacterium-mediated gene transfer systems for maize and wheat. Achieving effective transfer integration, and expression of transgenes using appropriate gene constructs promotes understanding of the process of gene transfer and the nature of transgenic plants. In addition the manual introduces readers to the basics of plant genome analysis and its application to the analysis of both maize and wheat transgenic plants. Also covered in this manual are the isolation, digestion, electrophoresis, and transfer of DNA to blots; the non-radioactive hybridization and detection technology in use at CIMMYT, and the application of polymerase chain reaction (PCR) technology to the analysis of transgenic plants. To complete the coverage of the topic, information on phenotypic screening of transgenic plants in the biosafety greenhouses is also presented.
Format: PDF
Language: English
Publisher: CIMMYT
Serie: CIMMYT Applied Molecular Genetics Laboratory
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Handbook
Place of Publication: Mexico
Pages: viii, 141 pages
Agrovoc: ELECTROPHORESIS
Agrovoc: METHODS
Agrovoc: MILLING
Agrovoc: ELECTROPHORESIS
Agrovoc: METHODS
Agrovoc: MILLING


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  • Institutional
    Collection of CIMMYT institutional documents
  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP

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