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Fine mapping of leaf rust resistance gene Lrzh84 using expressed sequence tag and sequence-tagged site markers, and allelism with other genes on wheat chromosome 1b

Author: Yue Zhou
Author: Xianchun Xia
Author: He, Zhonghu
Author: Xing Li
Author: Zaifeng Li
Author: Daqun Liu
Year: 2013
ISSN: 0031-949X
Abstract: Zhou 8425B, possessing the leaf rust resistance gene LrZH84, is an elite wheat (Triticum aestivum) parental line in the Yellow-Huai Valley region of China. In the present study, 2,086 F2 plants derived from Zhou 8425B/Chinese Spring were used for fine mapping of LrZH84 with expressed sequence tag (EST) and sequence-tagged site (STS) markers. Seventy inter-simple sequence repeat EST and STS markers on 1BL were used to screen the two parents and resistant and susceptible bulks; those polymorphic were used to analyze the entire F2 population. Three EST markers (BF474863, BE497107, and CD373538) were closely linked to LrZH84, with genetic distances of 0.7, 0.7, and 1.7 cM, respectively. STS marker Hbsf-1 was developed from the sequences of polymerase chain reaction fragments amplified from EST marker BF474863. LrZH84 was 8.19 cM proximal to Lr44, but may be allelic to LrXi and LrG98 although they showed different reactions with some Puccinia triticina pathotypes.
Format: PDF
Language: English
Publisher: American Phytopathological Society (APS)
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Type: Article
Pages: 169-174
Issue: 2
Volume: 103
DOI: 10.1094/PHYTO-08-12-0186-R
Keywords: Allelism Test
Keywords: Genetic Mapping
Keywords: Molecular Markers
Publisher URI:
Agrovoc: ALLELES
Journal: Phytopathology

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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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