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Quantification of Bt δ-endotoxins in leaf tissues of tropical Bt maize populations

Author: Murenga, M.
Author: Danson, J.
Author: Mugo, S.
Author: Githiri, S.M.
Author: Wanjala, B.
Year: 2012
ISSN: 1684-5315 (On line)
URI: http://hdl.handle.net/10883/3340
Abstract: In Kenya, stem borers destroy an estimated 13.5% of farmers' annual maize harvest. Maize transformed using Bacillus thuringiensis (Bt) derived genes controls stem borers without negative effects to humans, livestock or the environment. The effectiveness and sustainability of Bt transgenic technology in the control of stem borers depends on the levels of concentration of the Bt ä-endotoxins in plant tissues. Kenya introduced Bt maize events to test the efficacy of Bt maize in controlling stem borers, and to develop high-yielding and locally adapted Bt maize germplasm for farmers. The objective of this study was to assess under greenhouse conditions the concentration levels of Bt ä-endotoxins in the leaf tissues of the parents, the F1, and the F2:3 populations of tropical maize, as a measure of stability and sustainability. Kenya introduced Bt maize events to test the efficacy of Bt maize in controlling stem borers, and to develop high-yielding and locally adapted Bt maize germplasm for farmers. The objective of this study was to assess under greenhouse conditions the concentration levels of Bt ä-endotoxins in the leaf tissues of the parents, the F1, and the F2:3 populations of tropical maize, as a measure of stability and sustainability. Two public Bt maize lines (Event 216 and Event 223) containing the cry1Ab
Format: PDF
Language: English
Publisher: Academic Journals
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Type: Article
Country focus: Kenya
Pages: 11181-11186
Issue: 51
Volume: 11
DOI: 10.5897/AJB11.3433
Keywords: Bt Maize
Keywords: Stem Borers
Keywords: Bt δ-Endotoxins
Keywords: Enzyme-Linked Immunosorbent Assay
Keywords: Dot Blot Analysis
Keywords: Cry1Ab
Agrovoc: BACILLUS THURINGIENSIS
Agrovoc: MAIZE
Agrovoc: STEM EATING INSECTS
Agrovoc: ENDOTOXINS
Agrovoc: ELISA
Journal: African Journal of Biotechnology


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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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