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High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

Author: Berraies, S.
Author: Gharbi, M.S.
Author: Belzile, F.
Author: Yahyaoui, A.
Author: Hajlaoui, M.R.
Author: Trifi, M.
Author: Jean, M.
Author: Rezgui, S.
Year: 2013
ISSN: 1684-5315
URI: http://hdl.handle.net/10883/3304
Abstract: Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of M. graminicola in Tunisia.
Language: English
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Type: Article
Pages: 1344-1349
Journal issue: 12
Journal: African Journal of Biotechnology
Journal volume: 12
DOI: 10.5897/AJB12.2299
Keywords: Durum wheat
Keywords: Genetic diversity
Keywords: microsatellites
Keywords: Mycosphaerella graminicola
Keywords: micro geographical scale
Keywords: Tunisia
Publisher URI: http://www.academicjournals.org/Ajb/abstracts/abs2013/20Mar/Berraies%20et%20al.htm


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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