Show simple item record

High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

Author: Berraies, S.
Author: Gharbi, M.S.
Author: Belzile, F.
Author: Yahyaoui, A.
Author: Hajlaoui, M.R.
Author: Trifi, M.
Author: Jean, M.
Author: Rezgui, S.
Year: 2013
ISSN: 1684-5315
Abstract: Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of M. graminicola in Tunisia.
Language: English
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Pages: 1344-1349
Journal issue: 12
Journal: African Journal of Biotechnology
Journal volume: 12
DOI: 10.5897/AJB12.2299
Keywords: Durum wheat
Keywords: Genetic diversity
Keywords: microsatellites
Keywords: Mycosphaerella graminicola
Keywords: micro geographical scale
Keywords: Tunisia
Publisher URI:

Files in this item


This item appears in the following Collection(s)

  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

Show simple item record