Show simple item record

Characterization of dense and erect panicle 1 gene (tadep1) located on common wheat group 5 chromosomes and development of allele-specific markers

Author: Liu Ya-Nan
Author: Xia Xian-Chun
Author: He, Zhonghu
Year: 2013
ISSN: 0496-3490
URI: http://hdl.handle.net/10883/3289
Abstract: Dense and erect panicle 1 (OsDep1) gene is an important QTL controlling yield associated traits such as panicle length, erect type, and grain density in rice. In the present study, full-length genomic DNA sequences of TaDep1 on common wheat group 5 chromosomes were cloned by homologous cloning approach based on the sequences of rice OsDep1. TaDep1 has five exons and four introns, similar to that of rice OsDep1. The coding sequences of TaDep1-A1, TaDep1-B1, and TaDep1-D1 were 918, 888, and 900 bp, encoding polypepetides of 305, 295, and 299 amino acids, respectively. Five allelic variants on TaDep1-A1 locus, four on TaDep1-B1 locus, and two on TaDep1-D1 locus were identified. Three pairs of complementary dominant markers and one codominant marker were developed based on the sequence polymorphisms presented in allelic variants of TaDep1-A1 and TaDep1-B1. The codominant marker dep19, which can accurately discriminate the allelic variants of TaDep1-B1c from those of TaDep1-B1a, TaDep1-B1b,and TaDep1-B1d, was developed from a 30 bp InDel of different allelic variants at the fifth exon of TaDep1-B1. No significant association was found among the yield associated traits such as thousand-kernel weight, plant height, panicle length, spikelet number and spikelet spacing in 406 cultivars, indicating that these genes have no significant effect on the yield-related traits in current Chinese wheat cultivars.
Format: PDF
Language: English
Publisher: Institute of Crop Sciences
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Pages: 589-598
Issue: 4
Volume: 39
DOI: 10.3724/SP.J.1006.2013.00589
Keywords: Dense and Erect Panicle1 (Dep1) Gene
Keywords: Allelic Variation
Keywords: Allele-Specific Markers
Publisher URI: http://211.155.251.148:8080/zwxb/EN/abstract/abstract5345.shtml
Agrovoc: WHEAT
Agrovoc: PANICLES
Agrovoc: GENES
Agrovoc: ALLELES
Agrovoc: GENETIC MARKERS
Journal: Acta Agronomica Sinica


Files in this item

Thumbnail

This item appears in the following Collection(s)

  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

Show simple item record