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Segregation analysis indicates that Puroindoline b-2 variants 2 and 3 are allelic in Triticum aestivum and that a revision to Puroindoline b-2 gene symbolization is indicated

Author: Hongwei Geng
Author: Beecher, B.S.
Author: Pumphrey, M.
Author: He Zhonghu
Author: Morris, C.F.
Year: 2013
ISSN: 0733-5210
URI: http://hdl.handle.net/10883/3279
Abstract: Genetic and kernel texture relationships between Puroindoline b-2 variants 2 and 3 have not been fully established in wheat (Triticum aestivum L.). Here, 480 F2 plants, derived from three hard spring wheat populations were used to test the segregation of Puroindoline b-2 (Pinb-2) variants 2 and 3. Chi-square analysis indicated that Pinb-2 variants 2 and 3 in all three F2 populations segregated as a single bi-allelic locus, with segregation ratios fitting a 1:2:1 ratio. Using 448 of the 480 plants derived from these three F2 populations, the average SKCS hardness index of plants homozygous for Pinb-2 variant 2 vs. those homozygous for variant 3 was not significantly different (67.5 vs. 67.9). Results indicated that plants with Pina-D1b/Pinb-D1a were on average 10.0 Single Kernel Characterization System (SKCS) hardness index units harder than those carrying the Pina-D1a/Pinb-D1b haplotype. In conclusion, Pinb-2 variants 2 and 3 are allelic and exert little effect on kernel texture in hard-kernel T. aestivum germplasm. Further, the designation of Pinb-2v2 and Pinb-2v3 should be changed to Pinb-B2a and Pinb-B2b, respectively. We propose that Pinb-2 variants 1 and 4 of Chinese Spring be designated Pinb-D2a and Pinb-A2a, respectively.
Language: English
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Type: Article
Pages: 61-66
Journal issue: 1
Journal: Journal of Cereal Science
Journal volume: 57
DOI: 10.1016/j.jcs.2012.09.015
Keywords: kernel hardness
Keywords: Bread wheat
Keywords: Puroindoline b-2 variants
Keywords: Segregation ratios
Publisher URI: http://www.sciencedirect.com/science/article/pii/S0733521012002214


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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