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Regeneración de maíces blancos subtropicales vía embriogénesis somática

Author: Hernandez-Garcia, C.M.
Author: Lopez-Peralta, C.
Author: Buenrostro-Nava, M.T.
Author: Cárdenas Soriano, E.
Author: Pellegrineschi, A.
Year: 2007
URI: http://hdl.handle.net/10883/3053
Abstract: Maize (Zea mays L.) is destined mainly for animal feed (yellow maizes) and in a smaller proportion for human consumption (white). The subtropical white maizes are important for humans. However, there are problems of regeneration, via somatic embryogenesis, that limit its genetic transformation. Therefore the objective of the present study was to evaluate somatic embryogenesis in nine lines of subtropical white maize. In the induction of somatic embryogenesis, 1, 2 and 5 mg L−1 of 2,4-D and Dicamba were evaluated. Lines 78, 395 and 444 produced 70.1 to 87.2% of embryogenic calluses, similar to the controls (67.2 to 74.7%). Lines 442 and 332 presented a mean induction rate of calluses of 48.4 to 60.6%, whereas 330, 202, 204 and 331 showed the lowest percentages (3.9 to 26.2%). Lines 330, 331 and the control 216×72 presented the highest percentages with Dicamba, whereas line 442 had its highest percentage with 2,4-D. The induction of the lines varies among doses. Line 395 regenerated 1.11 plants per callus, similar to the three controls (0.74-0.95 plants), while 442, 78 and 332 regenerated 0.67, 0.37 and 0.33. Rooted seedlings (92-99%) and acclimatization (95-100%) were obtained in lines 395, 442, 78 and 332. The number of fertile regenerated plants was 228, 187, 108 and 79 in lines 395, 442, 78 and 332. Four embryogenic lines of subtropical white maize (395, 442, 78 and 332) were identified with regeneration capacity of fertile plants in 17 weeks and with potential for being subjected to genetic transformation.
Format: PDF
Language: English
Language: Spanish
Publisher: Colegio de Postgraduados
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Alternative Title: Regeneration of subtropical white maize via somatic embryogenesis
Type: Article
Region: Global
Pages: 743-753
Issue: 7
Volume: 41
Keywords: 2,4-D
Keywords: Dicamba
Publisher URI: http://www.colpos.mx/agrocien/Bimestral/2007/oct-nov/art-4.pdf
Agrovoc: ZEA MAYS
Agrovoc: HISTOLOGY
Journal: Agrociencia


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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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