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Detection of Bacillus thuringiensis genes in transgenic maize by the PCR method and FTA paper technology

Author: Danson, J.W.
Author: Kimani, M.
Author: Mbogori, M.
Year: 2006
URI: http://hdl.handle.net/10883/3034
Abstract: We optimized the PCR method to detect genetically engineered Bacillus thuringiensis (Bt) maize in open quarantine fields in Kenya. Many factors affect the extraction of the DNA from plants, such as the amount of tissue available, the condition of the plant material, the numbers of steps involved in the extraction procedure, and the required purity needed. We tested the application of the FTA paper technology for field sampling whereby leaf materials are not removed and transported from the site. We also applied the PCR method, a technique that is widely used for detection of genetically modified organism (GMOs). We used primers specific to the Bt genes present in the transgenic maize to screen for the Bt genes Cry 1Ab and Cry 1Ba.
Language: English
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Type: Article
Region: Global
Pages: 2345-2349
Journal issue: 22
Journal: African Journal of Biotechnology
Journal volume: 5
Keywords: Genetically modified organism (GMO) detection
Keywords: Maize
Keywords: Bacillus thuringiensis (Bt)
Keywords: PCR method
Keywords: FTA paper technology
Publisher URI: http://www.academicjournals.org/AJB/abstracts/abs2006/16Nov/Danson%20et%20al.htm


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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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