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A novel homeobox-like gene associated with reaction to stripe rust and powdery mildew in common wheat

Author: Liu, D.
Author: Xia, X.-C.
Author: He, Zhonghu
Author: Xu, S.-C.
Year: 2008
URI: http://hdl.handle.net/10883/2680
Abstract: Stripe rust and powdery mildew, caused by Puccinia striiformis f. sp. tritici and Blumeria graminis f. sp. tritici, respectively, are severe diseases in wheat (Triticum aestivum) worldwide. In our study, differential amplification of a 201-bp cDNA fragment was obtained in a cDNA-amplified fragment length polymorphism (AFLP) analysis between near-isogenic lines Yr10NIL and Avocet S, inoculated with P. striiformis f. sp. tritici race CYR29. A full-length cDNA (1,357 bp) of a homeobox-like gene, TaHLRG (GenBank accession no. EU385606), was obtained in common wheat based on the sequence of GenBank accession AW448633 with high similarity to the above fragment. The genomic DNA sequence (2,396 bp) of TaHLRG contains three exons and two introns. TaHLRG appeared to be a novel homeobox-like gene, encoding a protein with a predicted 66-amino-acid homeobox domain. It was involved in race-specific responses to stripe rust in real-time quantitative polymerase chain reaction (PCR) analyses with Yr9NIL, Yr10NIL, and Avocet S. It was also associated with adult-plant resistance to stripe rust and powdery mildew based on the field trials of doubled haploid lines derived from the cross Bainong 64/Jingshuang 16 and two F2:3 populations from the crosses Lumai 21/Jingshuang 16 and Strampelli/Huixianhong. A functional marker, THR1 was developed based on the sequence of TaHLRG and located on chromosome 6A using a set of Chinese Spring nulli-tetrasomic lines.
Format: PDF
Language: English
Publisher: American Phytopathological Society (APS)
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Region: Global
Pages: 1291-1296
Issue: 12
Volume: 98
DOI: 10.1094/PHYTO-98-12-1291
Agrovoc: WHEAT
Agrovoc: RUSTS
Agrovoc: POWDERY MILDEWS
Agrovoc: AMPLIFIED FRAGMENT LENGTH POLYMORPHISM
Agrovoc: DISEASE RESISTANCE
Journal: Phytopathology


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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