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Molecular marker-assisted selection of DH plants conferring genes resistant to powdery mildew in wheat (Triticum aestivum L.)

Author: Luo ying-Hao
Author: Chen Xin-Min
Author: Xia Lan-qin
Author: Chen Xiao
Author: He, Zhonghu
Author: Ren Zheng-Long
Year: 2005
ISSN: 0496-3487
URI: http://hdl.handle.net/10883/2455
Abstract: Powdery mildew , caused by Blumeria graminis f1 sp1 tritici , is one of the most destructive wheat diseases worldwide1 Using molecular markers to pyramid more effective resistance genes is one way to sustain the resistance of wheat cultivars1 The objective of this study was to identify the resistance gene pyramided in DH plants by molecular markers1 A total of 49 DH plants derived from wheat ×maize crosses and potentially containing the multiple resistance genes were tested by the STS2PCR marker of Pm4b , SCAR2PCR marker of Pm13 & PmV , and theα2amylase isoenzyme marker of Pm121 The results showed that seven plants conferred with Pm4b + Pm12 + PmV , one plant with Pm4b + Pm13 + PmV , three plants with Pm12 + Pm4b , six plants with Pm4b + PmV , two plants with Pm12 + PmV , two plants with Pm13 + Pm4b , respectively ( Table 1) 1 When using wheat ×maize crosses to produce resistance gene pyramided DH plants , it could produce more pyramided DH types through callus inducing.
Format: PDF
Language: Chinese
Publisher: Institute of Crop Sciences
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Country focus: China
Region: East Asia
Pages: 565-570
Issue: 5
Volume: 31
Keywords: Doubled Haploid
Keywords: Pyramid Resistance Gene
Agrovoc: WHEAT
Agrovoc: POWDERY MILDEWS
Agrovoc: HAPLOIDY
Agrovoc: MARKER-ASSISTED SELECTION
Agrovoc: GENETIC RESISTANCE
Journal: Acta Agronomica Sinica


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  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP
  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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