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Enlisting wild grass genes to combat nitrification in wheat farming: a nature-based solution

Creator: Guntur Venkata Subbarao
Creator: Kishii, M.
Creator: Bozal-Leorri, A.
Creator: Ortiz-Monasterio, I.
Creator: Xiang Gao
Creator: Ibba, M.I.
Creator: Karwat, H.
Creator: Gonzalez-Moro, M.B.
Creator: Gonzalez-Murua, C.
Creator: Tadashi Yoshihashi
Creator: Tobita, S.
Creator: Kommerell, V.
Creator: Braun, H.J.
Creator: Iwanaga, M.
Year: 2021
URI: https://hdl.handle.net/10883/21645
Language: English
Publisher: National Academy of Sciences
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
Type: Article
Place of Publication: Washington, DC (USA)
Issue: 35
Volume: 118
DOI: 10.1073/pnas.2106595118
Keywords: Nitrification Inhibition
Keywords: Nitrogen Pollution
Keywords: Biological Nitrification Inhibition
Description: Active nitrifiers and rapid nitrification are major contributing factors to nitrogen losses in global wheat production. Suppressing nitrifier activity is an effective strategy to limit N losses from agriculture. Production and release of nitrification inhibitors from plant roots is termed “biological nitrification inhibition” (BNI). Here, we report the discovery of a chromosome region that controls BNI production in “wheat grass” Leymus racemosus (Lam.) Tzvelev, located on the short arm of the “Lr#3Nsb” (Lr#n), which can be transferred to wheat as T3BL.3NsbS (denoted Lr#n-SA), where 3BS arm of chromosome 3B of wheat was replaced by 3NsbS of L. racemosus. We successfully introduced T3BL.3NsbS into the wheat cultivar “Chinese Spring” (CS-Lr#n-SA, referred to as “BNI-CS”), which resulted in the doubling of its BNI capacity. T3BL.3NsbS from BNI-CS was then transferred to several elite high-yielding hexaploid wheat cultivars, leading to near doubling of BNI production in “BNI-MUNAL” and “BNI-ROELFS.” Laboratory incubation studies with root-zone soil from field-grown BNI-MUNAL confirmed BNI trait expression, evident from suppression of soil nitrifier activity, reduced nitrification potential, and N2O emissions. Changes in N metabolism included reductions in both leaf nitrate, nitrate reductase activity, and enhanced glutamine synthetase activity, indicating a shift toward ammonium nutrition. Nitrogen uptake from soil organic matter mineralization improved under low N conditions. Biomass production, grain yields, and N uptake were significantly higher in BNI-MUNAL across N treatments. Grain protein levels and breadmaking attributes were not negatively impacted. Wide use of BNI functions in wheat breeding may combat nitrification in high N input-intensive farming but also can improve adaptation to low N input marginal areas.
Agrovoc: NITROGEN
Agrovoc: POLLUTION
Agrovoc: NITRIFICATION INHIBITORS
Agrovoc: GENETIC IMPROVEMENT
Agrovoc: WHEAT
ISSN: 1091-6490
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article number: e2106595118


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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