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Genetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea

Creator: Muchero, W.
Creator: Roberts, P.A.
Creator: Diop, N.N.
Creator: Drabo, I.
Creator: Cisse, N.
Creator: Close, T.J.
Creator: Satoru Muranaka
Creator: Boukar, O.
Creator: Ehlers, J.D.
Year: 2013
URI: https://hdl.handle.net/10883/21642
Language: English
Publisher: Public Library of Science
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
Type: Article
Place of Publication: San Francisco, CA (USA)
Issue: 7
Volume: 8
DOI: 10.1371/journal.pone.0070041
Description: The stay-green phenomenon is a key plant trait with wide usage in managing crop production under limited water conditions. This trait enhances delayed senescence, biomass, and grain yield under drought stress. In this study we sought to identify QTLs in cowpea (Vigna unguiculata) consistent across experiments conducted in Burkina Faso, Nigeria, Senegal, and the United States of America under limited water conditions. A panel of 383 diverse cowpea accessions and a recombinant inbred line population (RIL) were SNP genotyped using an Illumina 1536 GoldenGate assay. Phenotypic data from thirteen experiments conducted across the four countries were used to identify SNP-trait associations based on linkage disequilibrium association mapping, with bi-parental QTL mapping as a complementary strategy. We identified seven loci, five of which exhibited evidence suggesting pleiotropic effects (stay-green) between delayed senescence, biomass, and grain yield. Further, we provide evidence suggesting the existence of positive pleiotropy in cowpea based on positively correlated mean phenotypic values (0.34< r <0.87) and allele effects (0.07< r <0.86) for delayed senescence and grain yield across three African environments. Three of the five putative stay-green QTLs, Dro-1, 3, and 7 were identified in both RILs and diverse germplasm with resolutions of 3.2 cM or less for each of the three loci, suggesting that these may be valuable targets for marker-assisted breeding in cowpea. Also, the co-location of early vegetative delayed senescence with biomass and grain yield QTLs suggests the possibility of using delayed senescence at the seedling stage as a rapid screening tool for post-flowering drought tolerance in cowpea breeding. BLAST analysis using EST sequences harboring SNPs with the highest associations provided a genomic context for loci identified in this study in closely related common bean (Phaseolus vulgaris) and soybean (Glycine max) reference genomes.
Agrovoc: QUANTITATIVE TRAIT LOCI
Agrovoc: SINGLE NUCLEOTIDE POLYMORPHISM
Agrovoc: CHROMOSOME MAPPING
Agrovoc: PLANT GENETICS
Agrovoc: DROUGHT
Agrovoc: GENOMICS
Agrovoc: PLANT BREEDING
Elocator: 70041
ISSN: 1932-6203
Journal: PLoS ONE


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  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP

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