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An AFLP marker linked to the leaf rust resistance gene LrBi16 and test of allelism with Lr14a on chromosome arm7BL

Creator: Peipei Zhang
Creator: Huixin Zhou
Creator: Caixia Lan
Creator: Zaifeng Li
Creator: Daqun Liu
Year: 2015
Language: English
Publisher: Elsevier
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Type: Article
Place of Publication: Netherlands
Pages: 152-156
Issue: 2
Volume: 3
DOI: 10.1016/j.cj.2014.11.004
Keywords: Genetic Linkage
Keywords: Molecular Mapping
Keywords: Puccinia triticina
Description: Leaf rust (LR), caused by Puccinia triticina, is one of the most widespread diseases of common wheat (Triticum aestivum L.) worldwide. The LR resistance gene LrBi16 has been mapped on chromosome arm 7BL in Chinese wheat cultivar Bimai 16 and was closely linked to SSR loci Xcfa2257 and Xgwm344 with genetic distances of 2.8 cM and 2.9 cM, respectively. In the present study, a total of 304 AFLP primer pairs were used to screen Bimai 16 and Thatcher and resistant and susceptible DNA bulks. The polymorphic AFLP marker P-ATT/M-CGC173bp was used to genotype F2 and F3 populations to identify markers more closely linked to LrBi16. Marker P-ATT/M-CGC173bp was tightly linked to LrBi16 with a genetic distance of 0.5 cM. As LrBi16 was mapped near the Lr14a locus, 809 F2 plants from the Bimai 16/RL6013 (Lr14a) cross were inoculated with the Pt pathotype FHNQ to test the allelism of Lr14a and LrBi16. All of the F2 plants were resistant to FHNQ (IT between ; and 2), suggesting that Lr14a and LrBi16 are allelic.
Agrovoc: RUSTS
Agrovoc: WHEAT
ISSN: 2214-5141
Journal: The Crop Journal

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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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