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Effect of genotype on In vitro propagation of elite sugarcane (Saccharum Officinarum L.) varieties of Ethiopian sugar Estates

Creator: Abu, G.
Creator: Mekbib, F.
Creator: Chere, A.T.
Year: 2014
URI: https://hdl.handle.net/10883/21451
Language: English
Publisher: IJTEEE
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: India
Pages: 123-128
Issue: 6
Volume: 2
Keywords: Acclimatization
Publisher URI: https://www.ijteee.org/research-paper-publishing/month/june2014.html
Description: In Ethiopia, sugar industry is increasing at an alarming rate and is expected to play a significant role in poverty reduction. Thus, tissue culture has irreplaceable potential as it enables rapid and large scale production of disease free planting material and creates novel genetic variation for improvement of the varieties. The experiment was carried out on two elite sugarcane varieties using leaf explants to investigate the effect of genotype on callus initiation and subsequent in vitro plant regeneration. The two varieties showed a statistically different response to various hormones and hormonal combinations with regard to most of the parameters measured. For callus initiation, vars. B52-298 and NCO-334 performed best on 3 mg/l and 2 mg/l of 2, 4-D respectively. 2 mg/l BA and 1 mg/l BA are better concentration of hormones for better shoot performance for B52-298 and NCO-334 variety respectively and hence no need of hormonal combination. Roots perform better on 1/2MS medium supplemented with 4 mg/l NAA for NCO-334 and 1mg/l IBA for B52-298 variety. The finding of the study is very important as it serves as a baseline for undertaking comprehensive sugarcane tissue culture research and other activities.
Agrovoc: AUXINS
Agrovoc: CALLUS
Agrovoc: CYTOKININS
Agrovoc: EXPLANTS
Agrovoc: GENOTYPES
Agrovoc: ROOTS
Agrovoc: SHOOTS
ISSN: 2347-4289
Journal: International Journal of Technology Enhancements and Emerging Engineering Research


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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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