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Resistance to barley scald (Rhynchosporium secalis)in the ethiopian donor lines 'Steudelli' and 'Jet', analyzed by partial least squares regression and interval mapping

Creator: Bjornstad, A.
Creator: Gronnerod, S.
Creator: Key, J.M.
Creator: Tekauz, A.
Creator: Crossa, J.
Creator: Martens, H.
Year: 2004
URI: https://hdl.handle.net/10883/21384
Language: English
Publisher: Mendelian Society of Lund
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: Lund, Sweden
Pages: 166-179
Issue: 2
Volume: 141
DOI: 10.1111/j.1601-5223.2004.01817.x
Publisher URI: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1601-5223.2004.01817.x
Description: The resistance of barley (Hordeum vulgare L.) to Rhynchosporium secalis (scald) has been investigated in two crosses between the susceptible cv. ‘Ingrid’ and two resistant Ethiopian landraces, ‘Steudelli’ and ‘Jet’. Doubled haploids were inoculated in replicated tests using two isolates of R. secalis, ‘4004’ and ‘WRS1872’. Expression of resistance differed widely between replicated tests. AFLP, SSR and RFLP markers were used to develop chromosome maps. Results have been analysed using partial least squares regression (PLSR) and interval mapping. In PLSR the major covariance structures or ‘latent variables’ between X (markers) and Y (isolates, tests) are modelled as principal components and their optimal number determined by cross‐validation. In ‘Steudelli’ two QTL were detected, one on each of chromosomes 3H and 7H, in 4 out of 5 tests, while in ‘Jet’ only one (different) allele at the 3H locus was found. The validated R2 varied between 11.0% and 64.9% in the replicated tests with ‘4004’.With isolate ‘WRS1872’ the 7H locus and another 3H locus were detected. By interval mapping the QTL detected were less stable and generally gave lower R2 values than PLSR. PLSR does not depend on maps, but interval mapping based on values predicted by PLSR had R2 around 90%. It is suggested that PLSR may be a useful tool in QTL analysis.
Agrovoc: BARLEY
Agrovoc: RHYNCHOSPORIUM SECALIS
Agrovoc: STATISTICAL METHODS
Agrovoc: AMPLIFIED FRAGMENT LENGTH POLYMORPHISM
Agrovoc: RESTRICTION FRAGMENT LENGTH POLYMORPHISM
Agrovoc: MICROSATELLITES
ISSN: 1601-5223
Journal: Hereditas


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    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP

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