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SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat

Creator: Dreisigacker, S.
Creator: Yonggui Xiao
Creator: Sehgal, D.
Creator: Guzman, C.
Creator: He Zhonghu
Creator: Xianchun Xia
Creator: Peña-Bautista, R.J.
Year: 2020
URI: https://hdl.handle.net/10883/21099
Language: English
Publisher: Public Library of Science
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
Type: Article
Country focus: China
Place of Publication: San Francisco, CA (USA)
Issue: 5
Volume: 15
DOI: 10.1371/journal.pone.0233056
Description: The content and composition of seed storage proteins is largely responsible for wheat end-use quality. They mainly consist of polymeric glutenins and monomeric gliadins. According to their electrophoretic mobility, gliadins and glutenins are subdivided into several fractions. Glutenins are classified as high molecular weight or low molecular weight glutenin subunits (HMW-GSs and LMW-GSs, respectively). LMW-GSs are encoded by multigene families located at the orthologous Glu-3 loci. We designed a set of 16 single-nucleotide polymorphism (SNP) markers that are able to detect SDS-PAGE alleles at the Glu-A3 and Glu-B3 loci. The SNP markers captured the diversity of alleles in 88 international reference lines and 27 Mexican cultivars, when compared to SDS-PAGE and STS markers, however, showed a slightly larger percent of multiple alleles, mainly for Glu-B3. SNP markers were then used to determine the Glu-1 and Glu-3 allele composition in 54 CIMMYT historical lines and demonstrated to be useful tool for breeding programs to improve wheat end product properties.
Agrovoc: WHEAT
Agrovoc: SINGLE NUCLEOTIDE POLYMORPHISM
Agrovoc: BREAD
Agrovoc: GENOTYPES
Agrovoc: GLUTEN
Related Datasets: https://figshare.com/articles/journal_contribution/Sequence_alignment_of_designed_KASP_assays_associated_to_i_Glu-A3_i_and_i_Glu-B3_i_alleles_/12289829
Elocator: 0233056
ISSN: 1932-6203
Journal: PLoS ONE


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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