Show simple item record

GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm

Author: Sukhwinder-Singh
Author: Sehgal, D.
Author: Satish Kumar
Author: Mian A. R. Arif
Author: Vikram, P.
Author: Sansaloni, C.P.
Author: Fuentes Dávila, G.
Author: Ortiz, C.
Year: 2020
ISSN: 2045-2322 (Print)
URI: https://hdl.handle.net/10883/20840
Format: PDF
Language: English
Publisher: Nature Publishing Group
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: London (United Kingdom)
Issue: 1
Issue: art. 5999
Volume: 10
DOI: 10.1038/s41598-020-62711-7
Description: This study was initiated to identify genomic regions conferring resistance to Karnal Bunt (KB) disease in wheat through a genome-wide association study (GWAS) on a set of 179 pre-breeding lines (PBLs). A GWAS of 6,382 high-quality DArTseq SNPs revealed 15 significant SNPs (P-value <10−3) on chromosomes 2D, 3B, 4D and 7B that were associated with KB resistance in individual years. In particular, two SNPs (chromosome 4D) had the maximum R2 values: SNP 1114200 | F | 0–63:T > C at 1.571 cM and R2 of 12.49% and SNP 1103052 | F | 0–61:C > A at 1.574 cM and R2 of 9.02%. These two SNPs displayed strong linkage disequilibrium (LD). An in silico analysis of SNPs on chromosome 4D identified two candidate gene hits, TraesCS4D02G352200 (TaNox8; an NADPH oxidase) and TraesCS4D02G350300 (a rhomboid-like protein belonging to family S54), with SNPs 1103052 | F | 0–61:C > A and 1101835 | F | 0–5:C > A, respectively, both of which function in biotic stress tolerance. The epistatic interaction analysis revealed significant interactions among 4D and 7B loci. A pedigree analysis of confirmed resistant PBLs revealed that Aegilops species is one of the parents and contributed the D genome in these resistant PBLs. These identified lines can be crossed with any elite cultivar across the globe to incorporate novel KB resistance identified on 4B.
Agrovoc: PLANT DISEASES
Agrovoc: GENETIC RESISTANCE
Agrovoc: SINGLE NUCLEOTIDE POLYMORPHISM
Agrovoc: MARKER-ASSISTED SELECTION
Agrovoc: WHEAT
Related Datasets: https://www.nature.com/articles/s41598-020-62711-7#Sec17
Journal: Nature Scientific Reports


Files in this item

Thumbnail

This item appears in the following Collection(s)

  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP
  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

Show simple item record