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QTL mapping and transcriptome analysis to identify differentially expressed genes induced by Septoria tritici blotch disease of wheat

Author: Odilbekov, F.
Author: Xinyao He
Author: Armoniené, R.
Author: Saripella, G.V.
Author: Henriksson, T.
Author: Singh, P.K.
Author: Chawade, A.
Year: 2019
ISSN: 2073-4395
URI: https://hdl.handle.net/10883/20546
Abstract: Resistance to Septoria tritici blotch (STB) is an economically important trait in many wheat-breeding programs across the world. Several quantitative trait loci (QTL) for STB resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genes/QTL and determine the underlying resistance mechanism. In this work, we integrated QTL mapping and transcriptome profiling to identify candidate genes underlying QTL associated with STB resistance in bread wheat at the seedling stage. The results revealed four QTL on chromosomes 1BS, 1BL, 3AS and 3DL for STB resistance. Among these, two QTL on 2BL and 3DL were mapped for chlorosis, necrosis and pycnidia while the other two on 1BS and 3AS were associated with necrosis and pycnidia. Among the four identified QTL, genes were identified in three QTL (1BS, 2BL and 3DL). In total, 238 differentially expressed genes (DEGs) were localized in 1BS, 16 DEGs in 2BL and 80 DEGs in 3DL QTL region respectively. F-box protein, NBS-LRR disease resistance genes and receptor-like protein kinase were the most over-represented. The results emphasize the importance of integrating QTL and transcriptome analysis to accelerate the identification of key genes underlying the traits of interest.
Format: PDF
Language: English
Publisher: MDPI
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: Basel (Switzerland)
Pages: art. 510
Issue: 9
Volume: 9
DOI: 10.3390/agronomy9090510
Agrovoc: SEPTORIA
Agrovoc: MYCOSPHAERELLA GRAMINICOLA
Agrovoc: GENES
Agrovoc: DISEASE RESISTANCE
Agrovoc: WHEAT
Agrovoc: QUANTITATIVE TRAIT LOCI
Related Datasets: https://www.mdpi.com/2073-4395/9/9/510/s1
Journal: Agronomy


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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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