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Hydrogen peroxide prompted lignification affects pathogenicity of hemi-biotrophic pathogen Bipolaris sorokiniana to wheat

Author: Poudel, A.
Author: Sudhir Navathe
Author: Chand, R.
Author: Vinod Kumar Mishra
Author: Singh, P.K.
Author: Joshi, A.K.
Year: 2019
ISSN: 1598-2254
ISSN: 2093-9280 (Online)
Abstract: Spot blotch caused by Bipolaris sorokiniana has spread to more than 9 million ha of wheat in the warm, humid areas of the Eastern Gangetic Plains (EGP) of South Asia and is a disease of major concern in other similar wheat growing regions worldwide. Differential lignin content in resistant and susceptible genotypes and its association with free radicals such as hydrogen peroxide (H2O2), superoxide (O2−) and hydroxyl radical (OH−) were studied after inoculation under field conditions for two consecutive years. H2O2 significantly influenced lignin content in flag leaves, whereas there was a negative correlation among lignin and H2O2 to the Area Under Disease Progress Curve (AUDPC). The production of H2O2 was higher in the resistant genotypes than susceptible ones. The O2− and OH− positively correlated with AUDPC but negatively with lignin content. This study illustrates that H2O2 has a vital role in prompting lignification and thereby resistance to spot blotch in wheat. We used cluster analysis to separate the resistant and susceptible genotypes by phenotypic and biochemical traits. H2O2 associated lignin production significantly reduced the number of appressoria and penetration pegs. We visualized the effect of lignin in disease resistance using differential histochemical staining of tissue from resistant and susceptible genotypes, which shows the variable accumulation of hydrogen peroxide and lignin around penetration sites.
Format: PDF
Language: English
Publisher: Korean Society of Plant Pathology
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: Korea
Pages: 287-300
Issue: 4
Volume: 35
DOI: 10.5423/PPJ.OA.09.2018.0180
Agrovoc: LIGNINS
Agrovoc: WHEAT
Journal: Plant Pathology Journal

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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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