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Doubled haploid technology for line development in maize: technical advances and prospects

Author: Chaikam, V.
Author: Molenaar, W.
Author: Melchinger, A.E.
Author: Prasanna, B.M.
Year: 2019
ISSN: 0040-5752 (Print)
URI: https://hdl.handle.net/10883/20309
Abstract: Doubled haploid (DH) technology has become an integral part of many commercial maize breeding programs as DH lines offer several economic, logistic and genetic benefits over conventional inbred lines. Further, new advances in DH technology continue to improve the efficiency of DH line development and fuel its increased adoption in breeding programs worldwide. The established method for maize DH production covered in this review involves in vivo induction of maternal haploids by a male haploid inducer genotype, identification of haploids from diploids at the seed or seedling stage, chromosome doubling of haploid (D0) seedlings and finally, selfing of fertile D0 plants. Development of haploid inducers with high haploid induction rates and adaptation to different target environments have facilitated increased adoption of DH technology in the tropics. New marker systems for haploid identification, such as the red root marker and high oil marker, are being increasingly integrated into new haploid inducers and have the potential to make DH technology accessible in germplasm such as some Flint, landrace, or tropical material, where the standard R1-nj marker is inhibited. Automation holds great promise to further reduce the cost and time in haploid identification. Increasing success rates in chromosome doubling protocols and/or reducing environmental and human toxicity of chromosome doubling protocols, including research on genetic improvement in spontaneous chromosome doubling, have the potential to greatly reduce the production costs per DH line.
Format: PDF
Language: English
Publisher: Springer
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: Berlin (Germany)
Pages: 3227-3243
Issue: 12
Volume: 132
DOI: 10.1007/s00122-019-03433-x
Agrovoc: HAPLOIDY
Agrovoc: MAIZE
Agrovoc: PLANT BREEDING
Journal: Theoretical and Applied Genetics


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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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