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Introgression of the crtRB1 gene into quality protein maize inbred lines using molecular markers

Author: Li Liu
Author: Jeffers, D.
Author: Zhang, Y.
Author: Ding, M.
Author: Wei Chen
Author: Kang, M.S.
Author: Xingming Fan
Year: 2015
ISSN: 1380-3743
ISSN: 1572-9788
Abstract: Quality protein maize (QPM; Zea mays L.) has effectively enhanced levels of the amino acids, lysine, and tryptophan, over normal maize and provided balanced dietary protein for the health and development of monogastric animals and humans. However, as in normal maize, QPM varieties are low in provitamin A (ProVA), a precursor of vitamin A, which can lead to vitamin A deficiency in humans when maize is a significant part of their diet. In this study, maize inbred Hp321-1 carrying the favorable alleles crtRB1-5′TE-2 and crtRB1-3′TE-1 that can enhance levels of ProVA, was used as donor for improving ProVA in QPM inbred lines CML161 and CML171. Functional markers for identifying the favorable alleles crtRB1-5′TE-2 and crtRB1-3′TE-1 were used in foreground selection, and simple sequence repeat markers were used in background selection for the BC1F1, BC2F1, and BC2F2 generations. The background recovery rates were 77.4 and 84.5 % for CML161 and CML171 populations, respectively, in the BC1F1 generation, and 89.9 and 92.1 % in the BC2F2 generation. With foreground and background selection, the mean ProVA concentration has been improved from 1.60 µg g−1 in the parent of CML161 to 5.25 µg g−1 in its BC2F3 offspring, from 1.80 µg g−1 in the parent of CML171 to 8.14 µg g−1 in its BC2F3 offspring while maintaining similar QPM characteristics of the recurrent parents. The success from this study offers maize breeders a procedure for increasing ProVA in QPM lines, which will greatly mitigate vitamin A deficiency and protein-energy malnutrition in developing countries.
Format: PDF
Language: English
Publisher: Springer
Publisher: Springer Nature
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: United States
Pages: art. 154
Issue: 8
Volume: 35
DOI: 10.1007/s11032-015-0349-7
Keywords: Provitamin A
Keywords: Crtrb1 Gene
Keywords: Functional Markers
Keywords: Foreground Selection
Keywords: Background Selection
Agrovoc: GENES
Agrovoc: MAIZE
Related Datasets:
Journal: Molecular Breeding

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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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