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Markers linked to wheat stem rust resistance gene Sr11 effective to puccinia graminis f. sp. tritici Race TKTTF

Author: Nirmala, J.
Author: Chao, S.
Author: Olivera, P.
Author: Babiker, E.M.
Author: Abeyo, B.
Author: Tadesse, Z.
Author: Imtiaz, M.
Author: Talbert, L.
Author: Blake, N.K.
Author: Akhunov, E.
Author: Pumphrey, M.O.
Author: Jin, Y.
Author: Rouse, M.N.
Year: 2016
Year: 2016
ISSN: 0031-949X
ISSN: 1943-7684
URI: https://hdl.handle.net/10883/19718
Descriptors: Wheat
Descriptors: Puccinia Graminis
Descriptors: Genetic Resistance
Descriptors: Genetic Stocks
Abstract: Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although stem rust can be controlled by the use of genetic resistance, population dynamics of P. graminis f. sp. tritici can frequently lead to defeat of wheat stem rust resistance genes. P. graminis f. sp. tritici race TKTTF caused a severe epidemic in Ethiopia on Ug99-resistant ‘Digalu’ in 2013 and 2014. The gene Sr11 confers resistance to race TKTTF and is present in ‘Gabo 56’. We identified seven single-nucleotide polymorphism (SNP) markers linked to Sr11 from a cross between Gabo 56 and ‘Chinese Spring’ exploiting a 90K Infinium iSelect Custom beadchip. Five SNP markers were validated on a ‘Berkut’/‘Scalavatis’ population that segregated for Sr11, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two of the SNP markers, KASP_6BL_IWB10724 and KASP_6BL_IWB72471, were predictive of Sr11 among wheat genetic stocks, cultivars, and breeding lines from North America, Ethiopia, and Pakistan. These markers can be utilized to select for Sr11 in wheat breeding and to detect the presence of Sr11 in uncharacterized germplasm.
Abstract: Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although stem rust can be controlled by the use of genetic resistance, population dynamics of P. graminis f. sp. tritici can frequently lead to defeat of wheat stem rust resistance genes. P. graminis f. sp. tritici race TKTTF caused a severe epidemic in Ethiopia on Ug99-resistant ‘Digalu’ in 2013 and 2014. The gene Sr11 confers resistance to race TKTTF and is present in ‘Gabo 56’. We identified seven single-nucleotide polymorphism (SNP) markers linked to Sr11 from a cross between Gabo 56 and ‘Chinese Spring’ exploiting a 90K Infinium iSelect Custom beadchip. Five SNP markers were validated on a ‘Berkut’/‘Scalavatis’ population that segregated for Sr11, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two of the SNP markers, KASP_6BL_IWB10724 and KASP_6BL_IWB72471, were predictive of Sr11 among wheat genetic stocks, cultivars, and breeding lines from North America, Ethiopia, and Pakistan. These markers can be utilized to select for Sr11 in wheat breeding and to detect the presence of Sr11 in uncharacterized germplasm.
Language: English
Publisher: American Phytopathological Society
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Type: Article
Place: United States
Pages: 1352-1358
Journal issue: 11
Journal: Phytopathology
Journal volume: 106
DOI: 10.1094/PHYTO-04-16-0165-R
Audicence: Researchers
Country of Focus: North america, Ethiopia,pakistan


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    Wheat - breeding, phytopathology, physiology, quality, biotech

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