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Genetic mapping of resistance in hexaploid wheat for a quarantine disease

Author: Gurcharn S. Brar
Author: Fuentes Dávila, G.
Author: Xinyao He
Author: Sansaloni, C.P.
Author: Singh, R.P.
Author: Singh, P.K.
Year: 2018
ISSN: 1664-462X
ISSN: ESSN: 1664-462X
Descriptors: Karnal bunt
Descriptors: Genetic code
Descriptors: Hexaploidy
Descriptors: Wheats
Abstract: Karnal bunt (KB) of wheat, caused by Tilletia indica, is one of the greatest challenges to grain industry, not because of yield loss, but quarantine regulations that restrict international movement and trade of affected stocks. Genetic resistance is the best way to manage this disease. Although several different sources of resistance have been identified to date, very few of those have been subjected to genetic analyses. Understanding the genetics of resistance, characterization and mapping of new resistance loci can help in development of improved germplasm. The objective of this study was to identify and characterize resistance loci (QTL) in two independent recombinant inbred lines (RILs) populations utilizing different wheat lines as resistance donors. Elite CIMMYT wheat lines Blouk#1 and Huirivis#1 were used as susceptible female parents and WHEAR/KUKUNA/3/C80.1/3BATAVIA//2WBLL1 (WKCBW) and Mutus as moderately resistant male parents in Pop1 and Pop2 populations, respectively. Populations were evaluated for KB resistance in 2015–16 and 2016–17 cropping seasons at two seeding dates (total four environments) in Cd. Obregon, Mexico. Two stable QTL from each population were identified in each environment: QKb.cim-2B and QKb.cim-3D (Pop1), QKb.cim-3B1 and QKb.cim-5B2 (Pop2). Other than those four QTL, other QTL were detected in each population which were specific to environments: QKb.cim-5B1, QKb.cim-6A, and QKb.cim-7A (Pop1), QKb.cim-3B2, QKb.cim-4A1, QKb.cim-4A2, QKb.cim-4B, QKb.cim-5A1, QKb.cim-5A2, and QKb.cim-7A2 (Pop2). Among the four stable QTL, all but QKb.cim-3B1 were derived from the resistant parent. QKb.cim-2B and QKb.cim-3D in Pop1 and QKb.cim-3B1 and QKb.cim-5B2 in Pop2 explained 5.0–11.4% and 3.3–7.1% phenotypic variance, respectively. A combination of two stable QTL in each population reduced KB infection by 24–33%, respectively. Transgressive resistant segregants lines derived with resistance alleles from both parents in each population were identified. Single nucleotide polymorphism (SNP) markers flanking these QTL regions may be amenable to marker-assisted selection. The best lines from both populations (in agronomy, end-use quality and KB resistance) carrying resistance alleles at all identified loci, may be used for inter-crossing and selection of improved germplasm in future. Markers flanking these QTL may assist in selection of such lines.
Language: English
Publisher: Frontiers
Publisher: Frontiers Media
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the sutable license for that purpose.
Type: Article
Place: Switzerland
Pages: art. 1497
Journal: Frontiers in Plant Science
Journal volume: 9
DOI: 10.3389/fpls.2018.01497
Keywords: Grain Industry
Keywords: Karnal
dc.relation.CRP: Wheat
Agrovoc: WHEAT
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  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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