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Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers

Author: Edet, O.U.
Author: Kim, J.
Author: Okamoto, M.
Author: Hanada, K.
Author: Takeda, T.
Author: Kishii, M.
Author: Gorafi, Y.S.A
Author: Tsujimoto, H.
Year: 2018
URI: https://hdl.handle.net/10883/19501
Abstract: Background: The tertiary gene pool of bread wheat, to which Leymus racemosus belongs, has remained underutilized due to the current limited genomic resources of the species that constitute it. Continuous enrichment of public databases with useful information regarding these species is, therefore, needed to provide insights on their genome structures and aid successful utilization of their genes to develop improved wheat cultivars for effective management of environmental stresses. Results: We generated de novo DNA and mRNA sequence information of L. racemosus and developed 110 polymorphic PCR-based markers from the data, and to complement the PCR markers, DArT-seq genotyping was applied to develop additional 9990 SNP markers. Approximately 52% of all the markers enabled us to clearly genotype 22 wheat-L. racemosus chromosome introgression lines, and L. racemosus chromosome-specific markers were highly efficient in detailed characterization of the translocation and recombination lines analyzed. A further analysis revealed remarkable transferability of the PCR markers to three other important Triticeae perennial species: L. mollis, Psathyrostachys huashanica and Elymus ciliaris, indicating their suitability for characterizing wheat-alien chromosome introgressions carrying chromosomes of these genomes. Conclusion: The efficiency of the markers in characterizing wheat-L. racemosus chromosome introgression lines proves their reliability, and their high transferability further broadens their scope of application. This is the first report on sequencing and development of markers from L. racemosus genome and the application of DArT-seq to develop markers from a perennial wild relative of wheat, marking a paradigm shift from the seeming concentration of the technology on cultivated species. Integration of these markers with appropriate cytogenetic methods would accelerate development and characterization of wheat-alien chromosome introgression lines.
Format: PDF
Language: English
Publisher: BioMed Central
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: London, U.K
Issue: art. 18
Volume: 19
DOI: 10.1186/s12863-018-0603-1
Keywords: Molecular Markers
Keywords: DArT-Seq
Keywords: Genome Sequencing
Keywords: Chromosome Introgression Lines
Keywords: Salinity Stress
Keywords: Leymus racemosus
Keywords: Triticeae
Agrovoc: LEYMUS
Agrovoc: DNA SEQUENCE
Agrovoc: SOFT WHEAT
Agrovoc: GENOMICS
Agrovoc: SOFT WHEAT
Agrovoc: CHROMOSOMES
Agrovoc: GENETIC MARKERS
Agrovoc: OSMOTIC STRESS
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Journal: BMC Genetics


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  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP

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