Hybrids performance of doubled haploid lines derived from 10 tropical bi-parental maize populations evaluated in contrasting environments in Kenya

Abstract

The doubled haploid (DH) technology enables maize breeders to develop a large number of homozygous lines rapidly and test them in hybrid combinations early in the breeding cycle. The objectives of this study were to evaluate testcross performance of 556 DH lines derived from 10 diverse tropical backcross (BC1) populations, and to estimate variance components and broad-sense heritability under both well-watered and managed drought-stress conditions in Kenya. The 556 DH testcrosses were divided into six trials, with each trial comprising 84 to 126 entries and six commercial checks developed through conventional pedigree method. Trials were evaluated at 3 or 4 well-watered (WW) and two managed drought-stress (WS) sites in 2012 using an alpha lattice design with three replications per environment. Test crosses of the DH lines showed significant differences in grain yield and other agronomic traits. In the combined analysis across the WW locations, the top 10 DH testcrosses from each trial gave 0.6 to 32.7% higher grain yield than the best commercial check. Under managed drought-stress condition, the top 10 DH testcrosses from each trial gave 11.8 to 40.9% more grain yield than the best check. The best DH lines identified in the study could be used in tropical maize breeding programs in Africa for improving grain yield and drought-tolerance. Following evaluation in advanced testing and national performance trials (NPT), a total of 36 hybrids involving DH lines from this study were recommended for commercial cultivation in east and southern Africa.