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Allelic variation at the TaZds-A1 locus on wheat chromosome 2A and development of a functional marker in common wheat

Author: Dong Chang-hai
Author: Ma Zhi-ying
Author: Xia Xian-Chun
Author: Zhang Li-Ping
Author: He, Zhonghu
Year: 2012
ISSN: 2095-3119
URI: http://hdl.handle.net/10883/1906
Abstract: Ζ (zeta)-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds-A1a allele.
Format: PDF
Language: English
Publisher: Elsevier
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Region: Global
Pages: 1067-1074
Issue: 7
Volume: 11
DOI: 10.1016/S2095-3119(12)60099-9
Keywords: Functional Marker
Keywords: QTL
Keywords: Carotene Desaturase
Keywords: ZDS
Publisher URI: http://www.sciencedirect.com/science/article/pii/S2095311912600999
Agrovoc: TRITICUM AESTIVUM
Agrovoc: GENETIC MARKERS
Agrovoc: QUANTITATIVE TRAIT LOCI
Agrovoc: CAROTENOIDS
Journal: Journal of Integrative Agriculture


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  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP
  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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