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Allelic variation at the TaZds-A1 locus on wheat chromosome 2A and development of a functional marker in common wheat

Author: Dong Chang-hai
Author: Ma Zhi-ying
Author: Xia Xian-Chun
Author: Zhang Li-Ping
Author: He, Zhonghu
Year: 2012
ISSN: 2095-3119
Abstract: Ζ (zeta)-carotene desaturase (ZDS) is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment (YP) content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame (ORF) of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-A1a and TaZds-A1b, yielding 183- and 179-bp fragments in TaZds-A1a and TaZds-A1b genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid (DH) population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-A1b allele was 7.8% higher than that of 91 cultivars with TaZds-A1a allele.
Format: PDF
Language: English
Publisher: Elsevier
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Type: Article
Region: Global
Pages: 1067-1074
Issue: 7
Volume: 11
DOI: 10.1016/S2095-3119(12)60099-9
Keywords: Functional Marker
Keywords: QTL
Keywords: Carotene Desaturase
Keywords: ZDS
Publisher URI:
Journal: Journal of Integrative Agriculture

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This item appears in the following Collection(s)

  • Genetic Resources
    Genetic Resources including germplasm collections, wild relatives, genotyping, genomics, and IP
  • Wheat
    Wheat - breeding, phytopathology, physiology, quality, biotech

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