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Bulked sample analysis in genetics, genomics and crop improvement

Author: Cheng Zou
Author: Pingxi Wang
Author: Yunbi Xu
Year: 2016
URI: http://hdl.handle.net/10883/18829
Abstract: Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high-throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker-assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives.
Format: PDF
Language: English
Publisher: Wiley Open Access
Publisher: Association of Applied Biologists
Publisher: Society for Experimental Biology
Publisher: Blackwell Publishing
Copyright: CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.
Type: Article
Place of Publication: Oxford, United Kingdom
Pages: pages 1941-1955
Issue: 10
Volume: v. 14
DOI: 10.1111/pbi.12559
Keywords: Bulked Sample Analysis
Keywords: Bulked Segregant Analysis
Keywords: Microarrays
Keywords: Chips
Keywords: DNA-seq
Keywords: RNA-seq
Keywords: Protein-seq
Agrovoc: GENETICS
Agrovoc: SAMPLING
Agrovoc: DNA SEQUENCE
Agrovoc: RNA SEQUENCE
Agrovoc: BREEDING
Related Datasets: https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12559#support-information-section
Journal: Plant Biotechnology Journal


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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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