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Bulked sample analysis in genetics, genomics and crop improvement

Author: Cheng Zou
Author: Pingxi Wang
Author: Yunbi Xu
Year: 2016
Abstract: Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high-throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker-assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives.
Format: PDF
Language: English
Publisher: Wiley Open Access
Publisher: Association of Applied Biologists
Publisher: Society for Experimental Biology
Publisher: Blackwell Publishing
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Type: Article
Place of Publication: Oxford, United Kingdom
Pages: pages 1941-1955
Issue: 10
Volume: v. 14
DOI: 10.1111/pbi.12559
Keywords: Bulked Sample Analysis
Keywords: Bulked Segregant Analysis
Keywords: Microarrays
Keywords: Chips
Keywords: DNA-seq
Keywords: RNA-seq
Keywords: Protein-seq
Related Datasets:
Journal: Plant Biotechnology Journal

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  • Maize
    Maize breeding, phytopathology, entomology, physiology, quality, and biotech

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