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Development of a seed DNA-based genotyping system for marker-assisted selection in maize
Leaf collection from the field, labeling and tracking back to the source plants after genotyping are rate limiting steps in leaf DNA-based genotyping. In this study, an optimized genotyping method using endosperm DNA sampled ...
Development of a West African yam Dioscorea spp. core collection
Yams (Dioscorea spp.) are important crops in some West African locations. The West African yam collections held at the IITA were characterized using the standard descriptor list for this crop (IPGRI/IITA. 1997. Descriptors ...
Detection of Bacillus thuringiensis genes in transgenic maize by the PCR method and FTA paper technology
We optimized the PCR method to detect genetically engineered Bacillus thuringiensis (Bt) maize in open quarantine fields in Kenya. Many factors affect the extraction of the DNA from plants, such as the amount of tissue ...
Development of an STS marker tightly linked to Yr26 against wheat stripe rust using the resistance gene-analog polymorphism (RGAP) technique
The gene Yr26 confers resistance to all races of Puccinia striiformis f. sp. tritici (PST), the casual pathogen of wheat stripe rust in China. Here, we report development of a molecular marker closely linked to Yr26 using ...
Kurimwa kwembeu yechibage chehybrid neOpen Pollinated Varieties (OPVs)
(FAO; CIMMYT, 2006)