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Este estudio se realizó para validar el locus de carácter cuantitativo (QTL- quantitative trait locus) HAP-6A-13, el cual fue reportado con asociación a peso de grano en trigos harineros de primavera del Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT). Para realizar la validación se diseñaron dos marcadores de competencia alelo específica (KASP- kompetitive allele specific PCR) para los polimorfismos de un solo nucleótido (SNP) T/C y A/G integrados en el QTL. La asociación de los marcadores KASP con el peso de mil granos y rendimiento se verificó mediante estudio de asociación del genoma completo (GWAS) en cuatro colecciones de trigo. Los dos marcadores KASP mostraron asociación significativa con el peso de mil granos y rendimiento (P ≤ 0.05); adicionalmente, se generaron 10 familias endogámicas heterogéneas (HIF-heterogeneous inbred families) y se evaluó el peso de mil granos, rendimiento y otros caracteres morfométricos del grano. En contraste con los resultados de GWAS, en las evaluaciones de las HIF el efecto del QTL HAP-6A-13 fue significativo (P ≤ 0.05) sólo en una HIF para peso de mil granos y en tres HIF para longitud de grano, lo que demuestra la dificultad de validar un QTL de efecto menor en trigo.
This study was conducted to validate the quantitative trait locus (QTL) HAP-6A-13, which was reported to be associated to grain weight in Spring bread wheat from the International Maize and Wheat Improvement Center (CIMMYT). To perform the validation, two Kompetitive Allele Specific PCR (KASP) markers were designed for the T/C and A/G single nucleotide polymorphisms (SNPs) comprising the QTL. The association of KASP markers with thousand kernel weight and grain yield was verified by genome-wide association study (GWAS) in four wheat collections. The two KASP markers showed significant association with thousand kernel weight and grain yield (P ≤ 0.05); in addition, 10 heterogeneous inbred families (HIF) were generated and thousand kernel weight, grain yield and other morphometric traits of the grain were evaluated. In contrast to the GWAS results, in the HIF evaluations the effect of the QTL HAP-6A-13 was significant (P ≤ 0.05) only in one HIF for thousand grain weight, and in three HIF for grain length, which demonstrates the difficulty of validating a QTL of minor effect in wheat.
This study was conducted to validate the quantitative trait locus (QTL) HAP-6A-13, which was reported to be associated to grain weight in Spring bread wheat from the International Maize and Wheat Improvement Center (CIMMYT). To perform the validation, two Kompetitive Allele Specific PCR (KASP) markers were designed for the T/C and A/G single nucleotide polymorphisms (SNPs) comprising the QTL. The association of KASP markers with thousand kernel weight and grain yield was verified by genome-wide association study (GWAS) in four wheat collections. The two KASP markers showed significant association with thousand kernel weight and grain yield (P ≤ 0.05); in addition, 10 heterogeneous inbred families (HIF) were generated and thousand kernel weight, grain yield and other morphometric traits of the grain were evaluated. In contrast to the GWAS results, in the HIF evaluations the effect of the QTL HAP-6A-13 was significant (P ≤ 0.05) only in one HIF for thousand grain weight, and in three HIF for grain length, which demonstrates the difficulty of validating a QTL of minor effect in wheat.
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Journal
Revista Fitotecnia Mexicana
Journal volume
45
Journal issue
2
Article number
Place of Publication
Mexico
Publisher
Sociedad Mexicana de Fitogenetica
Related Datasets
CGIAR Initiatives
Initiative
Accelerated Breeding
Impact Area
Nutrition, health & food security
Action Area
Genetic Innovation
Donor or Funder
CGIAR Trust Fund