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Fu, L., Xiao, Y., Yan, J., Liu, J., Wen, W., Zhang, Y., Xia, X., & He, Z. (2019). Characterization of TaCOMT genes associated with stem lignin content in common wheat and development of a gene-specific marker. Journal of Integrative Agriculture, 18(5), 939-947. https://doi.org/10.1016/s2095-3119(18)61958-6
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Stem lignin content (SLC) in common wheat (Triticum aestivum L.) contributes to lodging resistance. Caffeic acid 3-O-methyltransferase (COMT) is a key enzyme involved in lignin biosynthesis. Characterization of TaCOMT genes and development of gene-specific markers could enable marker-assisted selection in wheat breeding. In the present study, the full-length genomic DNA (gDNA) sequences of TaCOMT genes located on chromosomes 3A, 3B, and 3D were cloned by homologous cloning. Two allelic variants, TaCOMT-3Ba and TaCOMT-3Bb, were identified and differed by a 222-bp insertion/deletion (InDel) in the 3´-untranslated region (3´-UTR). A co-dominant gene-specific marker based on this InDel was developed and designated as TaCOMT-3BM. A total of 157 wheat cultivars and advanced lines grown in four environments were used to validate the associations between allelic patterns and SLC. The SLC of cultivars with TaCOMT-3Ba was significantly (P<0.01) higher than that of those with TaCOMT-3Bb, and the marker TaCOMT-3BM could be effectively used in wheat breeding.
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Journal
Journal of Integrative Agriculture
Journal volume
18
Journal issue
5
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Place of Publication
China
Publisher
Elsevier