Fifty one Fusarium isolates from crown tissue across 32 locations of Central Anatolian plateau. The 51 isolates which represented 13 different species were assessed for Fusarium pathogenicity against the winter wheat Pehlivan. Virulence ratings were variable with F. culmorum, F. pseudograminearum and F. graminearum causing the greatest severity and reduced plant weight. A strong negative correlation between plant weight and disease severity, other species including F. subglutinans, F. oxysporum, F. equiseti, F. acuminatum, F. solani, F. verticilloides were weak pathogens on Pehlivan and under these conditions would not be considered as pathogens.