Type
Date
Corporate author
Editor
Illustrator
Producer
Photographer
Contributor
Writer
Translator
Journal Title
Journal ISSN
Volume Title
Access Rights
APA citation
Dhakate, P., Sehgal, D., Vaishnavi, S., Chandra, A., Singh, A., Raina, S. N., & Rajpal, V. R. (2022). Comprehending the evolution of gene editing platforms for crop trait improvement. Frontiers in Genetics, 13, 876987. https://doi.org/10.3389/fgene.2022.876987
ISO citation
Abstract
Description
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Numerous gene editing-based crop improvement technologies involving CRISPR/Cas platforms individually or in combination with next-generation sequencing methods have been developed that have revolutionized plant genome-editing methodologies. Initially, CRISPR/Cas nucleases replaced the earlier used sequence-specific nucleases (SSNs), such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), to address the problem of associated off-targets. The adaptation of this platform led to the development of concepts such as epigenome editing, base editing, and prime editing. Epigenome editing employed epi-effectors to manipulate chromatin structure, while base editing uses base editors to engineer precise changes for trait improvement. Newer technologies such as prime editing have now been developed as a “search-and-replace” tool to engineer all possible single-base changes. Owing to the availability of these, the field of genome editing has evolved rapidly to develop crop plants with improved traits. In this review, we present the evolution of the CRISPR/Cas system into new-age methods of genome engineering across various plant species and the impact they have had on tweaking plant genomes and associated outcomes on crop improvement initiatives.
Keywords
Citation
Copyright
CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose
Journal
Frontiers in Genetics
Journal volume
13
Journal issue
Article number
876987
Place of Publication
Switzerland
Publisher
Frontiers Media
Related Datasets
CGIAR Initiatives
Initiative
Accelerated Breeding
Impact Area
Nutrition, health & food security
Action Area
Genetic Innovation
Donor or Funder
CGIAR Trust Fund