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Xianming Chen

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Xianming Chen
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Xianming Chen
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0000-0002-9013-5974

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Now showing 1 - 6 of 6
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    Genome-wide association study of common resistance to rust species in tetraploid wheat
    (Frontiers Media SA, 2024) Marone, D.; Laidò, G.; Saccomanno, A.; Petruzzino, G.; Giaretta Azevedo, C.V.; De Vita, P.; Mastrangelo, A.M.; Gadaleta, A.; Ammar, K.; Bassi, F.; Meinan Wang; Xianming Chen; Rubiales, D.; Matny, O.; Steffenson, B.; Pecchioni, N.
    Publication
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    MARPLE, a point-of-care, strain-level disease diagnostics and surveillance tool for complex fungal pathogens
    (BioMed Central, 2019) Radhakrishnan, G.V.; Cook, N.M.; Bueno Sancho, V.; Lewis, C. M.; Persoons, A.; Debebe, A.; Heaton, M.; Davey, P.E.; Abeyo Bekele Geleta; Alemayehu, Y.; Badebo, A.; Barnett, M.; Bryant, R.; Chatelain, J.; Xianming Chen; Suomeng Dong; Henriksson, T.; Holdgate, S.; Justesen, A.F.; Kalous, J.; Zhensheng Kang; Laczny, S.; Legoff, J.P.; Lesch, D.; Richards, T.; Randhawa, H.S.; Thach, T.; Meinan Wang; Hovmoller, M.S.; Hodson, D.P.; Saunders, D.G.O.
    Background: Effective disease management depends on timely and accurate diagnosis to guide control measures. The capacity to distinguish between individuals in a pathogen population with specific properties such as fungicide resistance, toxin production and virulence profiles is often essential to inform disease management approaches. The genomics revolution has led to technologies that can rapidly produce high-resolution genotypic information to define individual variants of a pathogen species. However, their application to complex fungal pathogens has remained limited due to the frequent inability to culture these pathogens in the absence of their host and their large genome sizes. Results: Here, we describe the development of Mobile And Real-time PLant disEase (MARPLE) diagnostics, a portable, genomics-based, point-of-care approach specifically tailored to identify individual strains of complex fungal plant pathogens. We used targeted sequencing to overcome limitations associated with the size of fungal genomes and their often obligately biotrophic nature. Focusing on the wheat yellow rust pathogen, Puccinia striiformis f.sp. tritici (Pst), we demonstrate that our approach can be used to rapidly define individual strains, assign strains to distinct genetic lineages that have been shown to correlate tightly with their virulence profiles and monitor genes of importance. Conclusions: MARPLE diagnostics enables rapid identification of individual pathogen strains and has the potential to monitor those with specific properties such as fungicide resistance directly from field-collected infected plant tissue in situ. Generating results within 48 h of field sampling, this new strategy has far-reaching implications for tracking plant health threats.
    Publication
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    Specificity of a rust resistance suppressor on 7DL in the spring wheat cultivar Canthatch
    (American Phytopathological Society (APS), 2015) Talajoor, M.; Yue Jin; Anmin Wan; Xianming Chen; Bhavani, S.; Tabe, L.; Lagudah, E.; Li Huang
    The spring wheat ‘Canthatch’ has been shown to suppress stem rust resistance genes in the background due to the presence of a suppressor gene located on the long arm of chromosome 7D. However, it is unclear whether the suppressor also suppresses resistance genes against leaf rust and stripe rust. In this study, we investigated the specificity of the resistance suppression. To determine whether the suppression is genome origin specific, chromosome location specific, or rust species or race specific, we introduced 11 known rust resistance genes into the Canthatch background, including resistance to leaf, stripe, or stem rusts, originating from A, B, or D genomes and located on different chromosome homologous groups. F1 plants of each cross were tested with the corresponding rust race, and the infection types were scored and compared with the parents. Our results show that the Canthatch 7DL suppressor only suppressed stem rust resistance genes derived from either the A or B genome, and the pattern of the suppression is gene specific and independent of chromosomal location.
    Publication
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    Seedling and slow rusting resistance to stripe rust in Chinese common wheats
    (American Phytopathological Society (APS), 2006) Li, Z.F.; Xianchun Xia; Zhou, X.C.; Niu, Y.C.; He Zhonghu; Yong Zhang; Li, G.Q.; Anmin Wan; Desen Wang; Xianming Chen; Lu, Q.L.; Singh, R.P.
    Identification of seedling and slow stripe rust resistance genes is important for gene pyramiding, gene deployment, and developing slow-rusting wheat cultivars to control the disease. A total of 98 Chinese lines were inoculated with 26 pathotypes of Puccinia striiformis f. sp. tritici for postulation of stripe rust resistance genes effective at the seedling stage. A total of 135 wheat lines were planted at two locations to characterize their slow rusting responses to stripe rust in the 2003-2004 and 2004-2005 cropping seasons. Genes Yr2, Yr3a, Yr4a, Yr6, Yr7, Yr9, Yr26, Yr27, and YrSD, either singly or in combinations, were postulated in 72 lines, whereas known resistance genes were not identified in the other 26 accessions. The resistance genes Yr9 and Yr26 were found in 42 and 19 accessions, respectively. Yr3a and Yr4a were detected in two lines, and four lines may contain Yr6. Three lines were postulated to possess YrSD, one carried Yr27, and one may possess Yr7. Thirty-three lines showed slow stripe rusting resistance at two locations in both seasons.
    Publication
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    Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines
    (American Phytopathological Society (APS), 2005) Wang, Z.L.; Li, L.H.; He Zhonghu; Duan, X.Y.; Zhou, Y.L.; Xianming Chen; Lillemo, M.; Singh, R.P.; Wang, H.; Xianchun Xia
    Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a widespread wheat disease in China. Identification of race-specific genes and adult plant resistance (APR) is of major importance in breeding for an efficient genetic control strategy. The objectives of this study were to (i) identify genes that confer seedling resistance to powdery mildew in Chinese bread wheat cultivars and introductions used by breeding programs in China and (ii) evaluate their APR in the field. The results showed that (i) 98 of 192 tested wheat cultivars and lines appear to have one or more resistance genes to powdery mildew; (ii) Pm8 and Pm4b are the most common resistance genes in Chinese wheat cultivars, whereas Pm8 and Pm3d are present most frequently in wheat cultivars introduced from CIMMYT, the United States, and European countries; (iii) genotypes carrying Pm1, Pm3e, Pm5, and Pm7 were susceptible, whereas those carrying Pm12, Pm16, and Pm20 were highly resistant to almost all isolates of B. graminis f. sp. tritici tested; and (iv) 22 genotypes expressed APR. Our data showed that the area under the disease progress curve, maximum disease severity on the penultimate leaf, and the disease index are good indicators of the degree of APR in the field. It may be a good choice to combine major resistance genes and APR genes in wheat breeding to obtain effective resistance to powdery mildew.
    Publication
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    Wheat strip rust epidemic and virulence of Puccinia striiformis f.sp. tritici in China in 2002
    (American Phytopathological Society (APS), 2004) Anmin Wan; Zhao, Z.; Xianming Chen; He Zhonghu; Jin, S.; Jia, Q.; Yao, G.; Yang, J.; Wang, B.; Li, G.; Bi, Y.; Yuan, Z.
    In China, wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat and can cause severe yield losses when susceptible cultivars are grown and weather conditions are favorable for the disease. Wheat stripe rust most frequently affects the winter wheat growing areas in Northwest, Southwest, and North China, and the spring wheat growing areas in Northwest China. In the 2001-2002 growing season, a widespread stripe rust epidemic affected about 6.6 million hectares of wheat in 11 provinces: Si-chuan, Chongqing, eastern Gansu, southern and western Shaanxi, southern and central Ningxia, Yunnan, Guizhou, Hubei, Henan, southern and central Hebei, and Shandong. The epidemic could be attributed to relatively warm weather from November 2001 to March 2002, high frequencies of stripe rust races CYR31 and CYR32, and widely grown susceptible cultivars. Race CYR31 was virulent on the Chinese differential cultivars Trigo Eureka, Fulhard, Lutescens 128, Mentana, Virgilio, Abbondanza, Early Premium, Funo, Danish 1, Fengchan 3, Lovrin 13, Shui-yuan 11, Lovrin 10, and Hybrid 46. Race CYR32 had all the virulence factors of CYR31, plus virulences on Chinese differential cultivars Jubilejina 2 and Kangyin 655, i.e., CYR32 was virulent on all differential cultivars, except Zhong 4. When tested on the world and European differential and some other resistant genotypes, CYR32 was virulent on Chinese 166 (Yr1), Heines VII (Yr2, Yr25, and YrHVII), Vilmorin 23 (Yr3a and Yr4a), Heines Kolben (Yr6 and YrHK), Lee (Yr7, Yr22, and Yr23), Clement (Yr9, Yr25, YrCle), VPM1 (Yr17), Selkirk (Yr27), Anza (YrA), Carstens V (YrCV1, YrCV2, and YrCV3), Gaby (YrG), Strubes Dickkopf (Yr25), and Suwon 92/Omar (YrSO). Resistance genes in Triticum spelta album (Yr5), Zhong 4, and Moro (Yr10 and YrMor) were effective against all races identified.
    Publication