Person:
Pretorius, Z.

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Pretorius
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Pretorius, Z.

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  • Sources of stem rust resistance in wheat-alien introgression lines
    (American Phytopathological Society (APS), 2016) Rahmatov, M.; Rouse, M.N.; Steffenson, B.; Andersson, S.C.; Wanyera, R.; Pretorius, Z.; Houben, A.; Kumarse, N.; Bhavani, S.; Johansson, E.
    Stem rust is one of the most devastating diseases of wheat. Widely virulent races of the pathogen in the Ug99 lineage (e.g., TTKSK) are threatening wheat production worldwide; therefore, there is an urgent need to enhance the diversity of resistance genes in the crop. The objectives of this study were to identify new sources of resistance in wheat-alien introgression derivatives from Secale cereale, Leymus mollis, L. racemosus, and Thinopyrum junceiforme, postulate genes conferring the resistance, and verify the postulated genes by use of molecular markers. From seedling tests conducted in the greenhouse, the presence of seven known stem rust resistance genes (Sr7b, Sr8a, Sr9d, Sr10, Sr31, Sr36, and SrSatu) was postulated in the wheat-alien introgression lines. More lines possessed a high level of resistance in the field compared with the number of lines that were resistant at the seedling stage. Three 2R (2D) wheat-rye substitution lines (SLU210, SLU238, and SLU239) seemed likely to possess new genes for resistance to stem rust based on their resistance pattern to 13 different stem rust races but the genes responsible could not be identified. Wheat-rye, wheat-L. racemosus, and wheat-L. mollis substitutions or translocations with single and multiple interchanges of chromosomes, in particular of the B and D chromosomes of wheat, were verified by a combination of genomic in situ hybridization and molecular markers. Thus, the present study identified novel resistance genes originating from different alien introgressions into the wheat genome of the evaluated lines. Such genes may prove useful in enhancing the diversity of stem rust resistance in wheat against widely virulent pathogen races such as those in the Ug99 lineage.
    Publication
  • Potential for re-emergence of wheat stem rust in the United Kingdom
    (Nature Research, 2018) Lewis, C. M.; Persoons, A.; Bebber, D.; Kigathi, R.; Maintz, J.; Findlay, K.; Bueno Sancho, V.; Corredor-Moreno, P.; Harrington, S.A.; Ngonidzashe Kangara; Berlin, A.; Garcia, R.; German, S.E.; Hanzalova, A.; Hodson, D.P.; Hovmoller, M.S.; Huerta-Espino, J.; Imtiaz, M.; Mirza, J.I.; Justesen, A.F.; Niks, R.; Ali Omrani; Patpour, M.; Pretorius, Z.; Ramin Roohparvar; Sela, H.; Singh, R.P.; Steffenson, B.; Visser, B.; Fenwick, P.; Thomas, J.; Wulff, B.B.H.; Saunders, D.G.O.
    Wheat stem rust, a devastating disease of wheat and barley caused by the fungal pathogen Puccinia graminis f. sp. tritici, was largely eradicated in Western Europe during the mid-to-late twentieth century. However, isolated outbreaks have occurred in recent years. Here we investigate whether a lack of resistance in modern European varieties, increased presence of its alternate host barberry and changes in climatic conditions could be facilitating its resurgence. We report the first wheat stem rust occurrence in the United Kingdom in nearly 60 years, with only 20% of UK wheat varieties resistant to this strain. Climate changes over the past 25 years also suggest increasingly conducive conditions for infection. Furthermore, we document the first occurrence in decades of P. graminis on barberry in the UK . Our data illustrate that wheat stem rust does occur in the UK and, when climatic conditions are conducive, could severely harm wheat and barley production.
    Publication