Person: Machida, L.
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Machida
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L.
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Machida, L.
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0000-0002-0012-3997 8 results
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- REMATTOOL-R: a smart tool for identifying superior maize genotypes from multi-environment yield trials(Taylor and Francis, 2023) Machida, L.; Huerta Rodriguez, F.; Makumbi, D.; Tarusenga, M.
Publication - Evaluation of early-generation tropical maize testcrosses for grain-yield potential and weevil (Sitophilus zeamais Motschulsky) resistance(Elsevier, 2021) Sserumaga, J.P.; Makumbi, D.; Oikeh, S.O.; Otim, M.; Machida, L.; Bruce, A.Y.; Nhamucho, E.; Beyene, Y.; Mugo, S.N.
Publication - Grain-yield stability among tropical maize hybrids derived from doubled-haploid inbred lines under random drought stress and optimum moisture conditions(CSIRO, 2018) Sserumaga, J.P.; Beyene, Y.; Pillay, K.; Alois Kullaya; Oikeh, S.O.; Mugo, S.N.; Machida, L.; Ismail Ngolinda; Asea, G.; Justin Ringo; Otim, M.; Abalo, G.; Barnabas KiulaDrought is a devastating environmental stress in agriculture and hence a common target of plant breeding. A review of breeding progress on drought tolerance shows that, to a certain extent, selection for high yield in stress-free conditions indirectly improves yield in water-limiting conditions. The objectives of this study were to (i) assess the genotype × environment (GE) interaction for grain yield (GY) and other agronomic traits for maize (Zea mays L.) across East African agro-ecologies; and (ii) evaluate agronomic performance and stability in Uganda and Tanzania under optimum and random drought conditions. Data were recorded for major agronomic traits. Genotype main effect plus GE (GGE) biplot analysis was used to assess the stability of varieties within various environments and across environments. Combined analysis of variance across optimum moisture and random drought environments indicated that locations, mean-squares for genotypes and GE were significant for most measured traits. The best hybrids, CKDHH1097 and CKDHH1090, gave GY advantages of 23% and 43%, respectively, over the commercial hybrid varieties under both optimum-moisture and random-drought conditions. Across environments, genotypic variance was less than the GE variance for GY. The hybrids derived from doubled-haploid inbred lines produced higher GY and possessed acceptable agronomic traits compared with the commercial hybrids. Hybrid CKDHH1098 ranked second-best under optimum-moisture and drought-stress environments and was the most stable with broad adaptation to both environments. Use of the best doubled-haploids lines in testcross hybrids make-up, well targeted to the production environments, could boost maize production among farmers in East Africa.
Publication - Exploration of farmers' preferences and perceptions of maize varieties: implications on development and adoption of quality protein maize (QPM) varieties in Zimbabwe(Canadian Center of Science and Education, 2014) Machida, L.; Derera, J.; Tongoona, P.; Langyintuo, A.S.; MacRobert, J.Quality protein maize (QPM) technology is relatively new in Zimbabwe and farmer awareness of QPM was low. Participation of smallholder farmers in the development of QPM breeding objectives and dissemination strategies was solicited through participatory rural appraisal (PRA) techniques. Seventy two farmers participated; the farmers were involved in the Mother Baby Trial (MBT) projects in four selected villages from three districts of Zimbabwe. Data collection techniques included work-sharing, village or resource mapping, Venn diagramming, semi structured interviewing, matrix scoring and ranking and pairwise ranking. The results suggested that protein malnutrition was prevalent in the districts. Maize was the most important crop and farmers grew three types of maize, namely landrace (“Hickory King”), open pollinated varieties (OPV) and hybrid varieties all representing normal endosperm maize. Hybrids were dominant and produced mainly for sale, while “Hickory King”, although not supported by the formal seed system, continued to be produced for home consumption because of its superior taste, white kernel color, large kernel size, high kernel density, kernel hardness, and perceived weevil-resistance. Lateness and foliar disease susceptibility were the disadvantages of Hickory King. The ideal maize variety should be early-maturing, with a high yield potential, drought tolerant, foliar disease resistant and stem borer tolerant. For any QPM variety to be acceptable, farmers expected it to combine the agronomic attributes of hybrids and the grain quality characteristics of “Hickory King”, an “heirloom” variety. To effectively promote the adoption of QPM, the Agricultural Research and Extension (AREX) arm of government was the farmers' choice compared to other modes of information dissemination which were radio, television, newspaper, church NGO and councillor.
Publication - Maize combined insect resistance genomic regions and their co-localization with cell wall constituents revealed by tissue-specific QTL meta-analyses(Frontiers, 2018) Badji, A.; Otim, M.; Machida, L.; Odong, T.; Kwemoi, D.B.; Okii, D.; Agbahoungba, S.; Mwila, N.; Kumi, F.; Ibanda, A.; Mugo, S.N.; Kyamanywa, S.; Rubaihayo, P.Combinatorial insect attacks on maize leaves, stems, and kernels cause significant yield losses and mycotoxin contaminations. Several small effect quantitative trait loci (QTL) control maize resistance to stem borers and storage pests and are correlated with secondary metabolites. However, efficient use of QTL in molecular breeding requires a synthesis of the available resistance information. In this study, separate meta-analyses of QTL of maize response to stem borers and storage pests feeding on leaves, stems, and kernels along with maize cell wall constituents discovered in these tissues generated 24 leaf (LIR), 42 stem (SIR), and 20 kernel (KIR) insect resistance meta-QTL (MQTL) of a diverse genetic and geographical background. Most of these MQTL involved resistance to several insect species, therefore, generating a significant interest for multiple-insect resistance breeding. Some of the LIR MQTL such as LIR4, 17, and 22 involve resistance to European corn borer, sugarcane borer, and southwestern corn borer. Eleven out of the 42 SIR MQTL related to resistance to European corn borer and Mediterranean corn borer. There KIR MQTL, KIR3, 15, and 16 combined resistance to kernel damage by the maize weevil and the Mediterranean corn borer and could be used in breeding to reduce insect-related post-harvest grain yield loss and field to storage mycotoxin contamination. This meta-analysis corroborates the significant role played by cell wall constituents in maize resistance to insect since the majority of the MQTL contain QTL for members of the hydroxycinnamates group such as p-coumaric acid, ferulic acid, and other diferulates and derivates, and fiber components such as acid detergent fiber, neutral detergent fiber, and lignin. Stem insect resistance MQTL display several co-localization between fiber and hydroxycinnamate components corroborating the hypothesis of cross-linking between these components that provide mechanical resistance to insect attacks. Our results highlight the existence of combined-insect resistance genomic regions in maize and set the basis of multiple-pests resistance breeding.
Publication - Increasing genetic gains in maize through breeding for insect resistance(CIMMYT, 2017) Bruce, A.Y.; Machida, L.; Beyene, Y.; Mugo, S.N.
Publication - Developing maize varieties that adapt to climate change :the role of CIMMYT and partners in Africa(CIMMYT, 2016) Regasa, M.W.; Mugo, S.N.; Beyene, Y.; Makumbi, D.; Owino, F.; Abate, T.; Olsen, M.; Machida, L.; Suresh, L.M.; Prasanna, B.M.; Jumbo, M.B
Publication - Improving maize grain yield under drought stress and non-stress environments in Sub-Saharan Africa using marker-assisted recurrent selection(Crop Science Society of America (CSSA), 2016) Beyene, Y.; Semagn, K.; Crossa, J.; Mugo, S.N.; Atlin, G.; Tarekegne, A.T.; Meisel, B.; Sehabiague, P.; Vivek, B.; Oikeh, S.O.; Alvarado Beltrán, G.; Machida, L.; Olsen, M.; Prasanna, B.M.; Banziger, M.In marker-assisted recurrent selection (MARS), a subset of molecular markers significantly associated with target traits of interest are used to predict the breeding value of individual plants, followed by rapid recombination and selfing. This study estimated genetic gains in grain yield (GY) using MARS in 10 biparental tropical maize (Zea may L.) populations. In each population, 148 to 184 F2:3 (defined as C0) progenies were derived, crossed with a single-cross tester, and evaluated under water-stressed (WS) and well-watered (WW) environments in sub- Saharan Africa (SSA). The C0 populations were genotyped with 190 to 225 single-nucleotide polymorphism (SNP) markers. A selection index based on marker data and phenotypic data was used for selecting the best C0 families for recombination. Individual plants from selected families were genotyped using 55 to 87 SNPs tagging specific quantitative trait loci (QTL), and the best individuals from each cycle were either intercrossed (to form C1) or selfed (to form C1S1 and C1S2). A genetic gain study was conducted using test crosses of lines from the different cycles F1 and founder parents. Test crosses, along with five commercial hybrid checks were evaluated under four WS and four WW environments. The overall gain for GY using MARS across the 10 populations was 105 kg ha−1 yr−1 under WW and 51 kg ha−1 yr−1 under WS. Across WW environments, GY of C1S2–derived hybrids were 8.7, 5.9, and 16.2% significantly greater than those of C0, founder parents, and commercial checks, respectively. Results demonstrate the potential of MARS for increasing genetic gain under both drought and optimum environments in SSA.
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