Person: Xinhai Li
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Xinhai Li
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Xinhai Li
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- ZmADF5, a maize actin-depolymerizing factor conferring enhanced drought tolerance in maize(MDPI, 2024) Bojuan Liu; Nan Wang; Ruisi Yang; Xiaonan Wang; Ping Luo; Yong Chen; Fei Wang; Mingshun Li; Jianfeng Weng; Degui Zhang; Hongjun Yong; Jienan Han; Zhiqiang Zhou; Xuecai Zhang; Zhuanfang Hao; Xinhai Li
Publication - Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design(Institute of Crop Sciences, 2023) Ping Luo; Houwen Wang; Zhiyong Ni; Ruisi Yang; Fei Wang; Hongjun Yong; Lin Zhang; Zhiqiang Zhou; Wei Song; Mingshun Li; Jie Yang; Jianfeng Weng; Zhaodong Meng; Degui Zhang; Jienan Han; Yong Chen; Runze Zhang; Liwei Wang; Meng Zhao; Wenwei Gao; Xiaoyu Chen; Wenjie Li; Zhuanfang Hao; Junjie Fu; Xuecai Zhang; Xinhai Li
Publication - Pollen self-elimination CRISPR–Cas genome editing prevents transgenic pollen dispersal in maize(Cell Press, 2023) Honglin Wang; Xiantao Qi; Jinjie Zhu; Changlin Liu; Hongwei Fan; Xuecai Zhang; Xinhai Li; Qin Yang; Chuanxiao Xie
Publication - Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize(BioMed Central, 2021) Nan Wang; Cheng, Ming; Yong Chen; Bojuan Liu; Xiaonan Wang; Guojun Li; Yueheng Zhou; Ping Luo; Zhangying Xi; Hongjun Yong; Degui Zhang; Mingshun Li; Xuecai Zhang; San Vicente Garcia, F.M.; Zhuanfang Hao; Xinhai Li
Publication - Heterotic grouping of maize inbred lines using RFLP and SSR markers(Institute of Crop Sciences, 2001) Yuan, L.X.; Fun Jun-Hua; Shihuang Zhang; Liu Xin-Zhi; Peng Ze-Bin; Xinhai Li; Warburton, M.; Khairallah, M.M.
Publication - Genomic prediction across years in a maize doubled haploid breeding program to accelerate early-stage testcross testing(Springer, 2020) Nan Wang; Hui Wang; Ao Zhang; Yubo Liu; Diansi Yu; Zhuanfang Hao; Ilut, D.; Glaubitz, J.C.; Yanxin Gao; Jones, E.; Olsen, M.; Xinhai Li; San Vicente Garcia, F.M.; Prasanna, B.M.; Crossa, J.; Pérez-Rodríguez, P.; Xuecai Zhang
Publication - Applications of genotyping-by-sequencing (GBS) in maize genetics and breeding(Nature Publishing Group, 2020) Nan Wang; Yibing Yuan; Hui Wang; Diansi Yu; Yubo Liu; Ao Zhang; Gowda, M.; Nair, S.K.; Zhuanfang Hao; Yanli Lu; San Vicente Garcia, F.M.; Prasanna, B.M.; Xinhai Li; Xuecai Zhang
Publication - An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design(Nature Publishing Group, 2016) Yongping Zhao; Congsheng Zhang; Wenwen Liu; Wei Gao; Changlin Liu; Gaoyuan Song; Wen-Xue Li; Long Mao; Beijiu Cheng; Yunbi Xu; Xinhai Li; Chuanxiao XiePrecision DNA/gene replacement is a promising genome-editing tool that is highly desirable for molecular engineering and breeding by design. Although the CRISPR/Cas9 system works well as a tool for gene knockout in plants, gene replacement has rarely been reported. Towards this end, we first designed a combinatory dual-sgRNA/Cas9 vector (construct #1) that successfully deleted miRNA gene regions (MIR169a and MIR827a). The deletions were confirmed by PCR and subsequent sequencing, yielding deletion efficiencies of 20% and 24% on MIR169a and MIR827a loci, respectively. We designed a second structure (construct #2) that contains sites homologous to Arabidopsis TERMINAL FLOWER 1 (TFL1) for homology-directed repair (HDR) with regions corresponding to the two sgRNAs on the modified construct #1. The two constructs were co-transformed into Arabidopsis plants to provide both targeted deletion and donor repair for targeted gene replacement by HDR. Four of 500 stably transformed T0 transgenic plants (0.8%) contained replaced fragments. The presence of the expected recombination sites was further confirmed by sequencing. Therefore, we successfully established a gene deletion/replacement system in stably transformed plants that can potentially be utilized to introduce genes of interest for targeted crop improvement.
Publication - Zea mays (L.) P1 locus for cob glume color identified as a post-domestication selection target with an effect on temperate maize genomes(Elsevier, 2013) Chuanxiao Xie; Jianfeng Weng; Wenguo Liu; Cheng Zou; Zhuanfang Hao; Wenxue Li; Minshun Li; Xiaosen Guo; Gengyun Zhang; Yunbi Xu; Xinhai Li; Shihuang ZhangArtificial selection during domestication and post-domestication improvement results in loss of genetic diversity near target loci. However, the genetic locus associated with cob glume color and the nature of the genomic pattern surrounding it was elusive and the selection effect in that region was not clear. An association mapping panel consisting of 283 diverse modern temperate maize elite lines was genotyped by a chip containing over 55,000 evenly distributed SNPs. Ten-fold resequencing at the target region on 40 of the panel lines and 47 tropical lines was also undertaken. A genome-wide association study (GWAS) for cob glume color confirmed the P1 locus, which is located on the short arm of chromosome 1, with a ; log10P value for surrounding SNPs higher than the Bonferroni threshold ( < 0.001) when a mixed linear model (MLM) was implemented. A total of 26 markers were identified in a 0.78 Mb region surrounding the P1 locus, including 0.73 Mb and 0.05 Mb upstream and downstream of the P1 gene, respectively. A clear linkage disequilibrium (LD) block was found and LD decayed very rapidly with increasing physical distance surrounding the P1 locus. The estimates of ; and Tajima's D were significantly (P < 0.001) lower at both ends compared to the locus. Upon comparison of temperate and tropical lines at much finer resolution by resequencing (180-fold finer than chip SNPs), a more structured LD block pattern was found among the 40 resequenced temperate lines. All evidence indicates that the P1 locus in temperate maize has not undergone neutral evolution but has been subjected to artificial selection during post-domestication selection or improvement. The information and analytical results generated in this study provide insights as to how breeding efforts have affected genome evolution in crop plants.
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