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Sserumaga, J.P.

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Sserumaga
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J.P.
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Sserumaga, J.P.
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0000-0001-5152-2835

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2014 - 20183
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Start date: 2014 × End date: 2019 × AGROVOC: MAIZE ×

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Now showing 1 - 3 of 3
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    Correction to: genotype by environment interactions and agronomic performance of doubled haploids testcross maize (Zea mays L.) hybrids
    (Springer, 2018) Sserumaga, J.P.; Oikeh, S.O.; Mugo, S.N.; Asea, G.; Otim, M.; Beyene, Y.; Abalo, G.; Kikafunda, J.
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    Molecular characterization of tropical maize inbred lines using microsatellite DNA markers
    (Consiglio per la Ricerca e la sperimentazione in Agricoltura, Unità di Ricerca per la Maiscoltura, 2014) Sserumaga, J.P.; Makumbi, D.; Ji, H.; Njoroge, K.; Muthomi, J.; Chemining’wa, G.N.; Si-myung, L.; Asea, G.; Kim, H.
    An insight on diversity and relationships among germplasm is important in any breeding program for crop improvement. The main objectives of our study were to: (i) determine the level of genetic diversity within mid altitude maize inbred lines resistant to weevils, aflatoxin accumulation and drought, (ii) to suggest potential heterotic groups using their genetic structures and distance based on cluster analysis with the aim to generate broad based source germplasm for mid altitude maize breeding program with combined traits of importance against Aflatoxin accumulation. In this study, 25 SSR markers were used to finger print forty two maize inbred lines to assess the genetic diversity, genetic relationships, and their population structure. A total of 184 alleles were identified at all the loci with an average of 7.36 and a range between two and 19 alleles per locus. The major allele frequency varied from 0.17 to 0.90 with an average of 0.49 while the minor allele frequency varied from 0.10 to 0.83 with an average of 0.51. The gene diversity values varied from 0.18 to 0.92 with an average of 0.65. Average heterozygosity percentage of the inbred lines was 4%, ranging from 0% to 2%, indicating the low level of heterozygosity within the inbred lines. The average polymorphism information content (PIC) was 0.61. A dendrogram constructed using unweighted Neighbour Joining algorithm suggested three heterotic groups among the inbred lines. The three heterotic patterns based on the SSR markers need to be verified by field testing to confirm what appears to be promising alternative heterotic patterns. The fixed pattern detected using SSR markers could potentially contribute towards effective utilization of the inbred lines for the exploitation of heterosis and formation of genetically diverse sources population.
    Publication
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    Genotype by environment interactions and agronomic performance of doubled haploids testcross maize (Zea mays L.) hybrids
    (Springer, 2016) Sserumaga, J.P.; Oikeh, S.O.; Mugo, S.N.; Asea, G.; Otim, M.; Beyene, Y.; Abalo, G.; Kikafunda, J.
    In vivo production of maternal haploid plants and advancement in chromosome doubling technology has led to rapid production of doubled haploid homozygous lines. These in turn have boosted rapid advancement in most breeding programs. This has resulted in production of a large number of maize hybrids which need testing across production environments to select the most suitable hybrids for release and cultivation. The objective of this study was to assess the genotype 9 environment interactions (GE) for grain yield and other agronomic traits and evaluate the performance of 44 recently developed doubled haploids (DH) testcross hybrids along with six checks across five locations in Uganda. Significant mean squares for environment (E), genotype (G) and GE were observed for all studied traits. Environment explained 46.5 % of the total variance, while G and GE contributed 13.2 and 7.2 %, respectively. Genetic correlations among locations were high (0.999), suggesting little GE among environments. The 10 best testcross hybrids had a 49.2 %average grain yield advantage over the six checks at all locations. DH hybrids CKHDHH0887, CKDHH0878, CKDHH 0859, WM1210, CKDHH0858, and WM1214 were the most stable, across locations. The DH testcross hybrids produced higher grain yield and possessed acceptable agronomic traits compared to the commercial hybrids developed earlier. Use of the best DH testcross hybrids, well targeted to the production environments, could boost maize production among farmers.
    Publication