Person: Joshi, A.K.
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Joshi
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A.K.
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Joshi, A.K.
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0000-0003-4205-932911 results
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- Novel resistance loci for quantitative resistance to Septoria tritici blotch in Asian wheat (Triticum aestivum) via genome-wide association study(BioMed Central Ltd, 2024) Patial, M.; Sudhir Navathe; Xinyao He; Kamble, U.; Kumar, M.; Joshi, A.K.; Singh, P.K.
Publication - Assessment of Indian wheat germplasm for Septoria nodorum blotch and tan spot reveals new QTLs conferring resistance along with recessive alleles of Tsn1 and Snn3(Frontiers Media S.A., 2023) Sudhir Navathe; Xinyao He; Kamble, U.; Kumar, M.; Patial, M.; Gyanendra Singh; Singh, G.P.; Joshi, A.K.; Singh, P.K.
Publication - Juvenile heat tolerance in wheat for attaining higher grain yield by shifting to early sowing in October in South Asia(MDPI, 2021) Kumar, U.; Singh, R.P.; Dreisigacker, S.; Roder, M.S.; Crossa, J.; Huerta-Espino, J.; Mondal, S.; Crespo Herrera, L.A.; Singh, G.P.; Mishra, C.N.; Mavi, G.S.; Sohu, V.S.; Prasad, S.V.S.; Naik, R.; Misra, S.; Joshi, A.K.
Publication - Evaluations of genomic prediction and identification of new loci for resistance to stripe rust disease in wheat (Triticum aestivum L.)(Frontiers, 2021) Tomar, V.; Dhillon, G.S.; Singh, D.; Singh, R.P.; Poland, J.; Chaudhary, A.A.; Bhati, P.; Joshi, A.K.; Kumar, U.
Publication - Identification of QTLs for spot blotch resistance in two bi-parental mapping populations of wheat(MDPI, 2021) Gahtyari, N.C.; Roy, C.; Xinyao He; Roy, K.K.; Reza, M.M.A.; Hakim M.A.; Malaker, P.K.; Joshi, A.K.; Singh, P.K.
Publication - Wheat stripe rust virulence and varietal resistance in the foot hill Himalayas of Nepal(David Publishing, 2015) Sharma, S; Duveiller, E.; Chandra Bahadur Karki; Thapa, D.B.; Sharma, R.; Joshi, A.K.
Publication - Inheritance of terminal heat tolerance in two spring wheat crosses(Akadémiai Kiadó, 2013) Paliwal, R.; Arun, B.; Srivastava, J.P.; Joshi, A.K.
Publication - Introgression of the high grain protein gene Gpc-B1 in an elite wheat variety of Indo-Gangetic Plains through marker assisted backcross breeding(Elsevier, 2014) Vishwakarma, M.K.; Mishra, V.K.; Gupta, P.K.; Yadav, P.S.; Kumar, H.; Joshi, A.K.Grain protein content (GPC) in wheat has been a major trait of interest for breeders since it has enormous end use potential. In the present study, marker-assisted backcrossing (MABC) was successfully used to improve GPC in wheat cultivar HUW468. The genotype Glu269 was used as the donor parent for introgression of the gene Gpc-B1 that confers high GPC. In a segregating population, SSR marker Xucw108, with its locus linked to Gpc-B1 was used for foreground selection to select plants carrying Gpc-B1. Background selection, involving 86 polymorphic SSR markers dispersed throughout the genome, was exercised to recover the genome of HUW468. For eliminating linkage drag, markers spanning a 10 cM region around the gene Gpc-B1 were employed to select lines with a donor segment of the minimum size carrying the gene of interest. Improved lines had significantly higher GPC and displayed 88.4–92.3 per cent of the recurrent parent genome (RPG). For grain yield, selected lines were at par with the recurrent parent HUW468, suggesting that there was no yield penalty. The whole exercise of transfer of Gpc-B1 and reconstitution of the genome of HUW468 was completed within a period of two and half years (five crop cycles) demonstrating practical utility of MABC for developing high GPC lines in the background of any elite and popular wheat cultivar with relatively higher speed and precision. (http://creativecommons.org/licenses/by-nc-nd/3.0/).
Publication - Resistance to spot blotch in two mapping populations of common wheat is controlled by multiple QTL of minor effects(MDPI, 2018) Singh, P.K.; Xinyao He; Sansaloni, C.; Juliana, P.; Dreisigacker, S.; Duveiller, E.; Kumar, U.; Joshi, A.K.; Singh, R.P.Spot blotch (SB) is an important fungal disease of wheat in South Asia and South America. Host resistance is regarded as an economical and environmentally friendly approach of controlling SB, and the inheritance of resistance is mostly quantitative. In order to gain a better understanding on the SB resistance echanism in CIMMYT germplasm, two bi-parental mapping populations were generated, both comprising 232 F2:7 progenies. Elite CIMMYT breeding lines, BARTAI and WUYA, were used as resistant parents, whereas CIANO T79 was used as susceptible parent in both populations. The two populations were evaluated for field SB resistance at CIMMYT’s Agua Fria station for three consecutive years, from the 2012–2013 to 2014–2015 cropping seasons. Phenological traits like plant height (PH) and days to heading (DH) were also determined. Genotyping was performed using the DArTSeq genotyping-by-sequencing (GBS) platform, and a few D-genome specific SNPs and those for phenological traits were integrated for analysis. The most prominent quantitative trait locus (QTL) in both populations was found on chromosome 5AL at the Vrn-A1 locus, explaining phenotypic variations of 7–27%. Minor QTL were found on chromosomes 1B, 3A, 3B, 4B, 4D, 5B and 6D in BARTAI and on chromosomes 1B, 2A, 2D and 4B in WUYA, whereas minor QTL contributed by CIANO T79 were identified on chromosome 1B, 1D, 3A, 4B and 7A. In summary, resistance to SB in the two mapping populations was controlled by multiple minor QTL, with strong influence from Vrn-A1.
Publication - Introgression of a gene for high grain protein content (Gpc-B1) into two leading cultivars of wheat in Eastern Gangetic Plains of India through marker assisted backcross breeding(Academic Journals, 2015) Mishra, V.K.; Pushpendra Kumar Gupta; Balasubramaniam, A.; Chand, R.; Vasistha, N.K.; Vishwakarma, M.K.; Punam S. Yadav; Joshi, A.K.A wheat genotype named ‘PBW343+Gpc-B1+LR24’ containing Gpc-B1 gene linked with the DNA-based marker Xucw108, developed at Choudhary Charan Singh University (CCSU), Meerut, through marker-assisted selection, was used as the donor parent to transfer the gene (Gpc-B1) for high grain protein content (GPC) into two popular cultivars (HUW234 and HUW468) of Eastern Gangetic Plains (EGP) of India. In both the cultivars, Gpc-B1 gene was introgressed through marker-assisted backcross breeding (MABB) which involved the following three steps: (i) Foreground selection; (ii) Screening for the carrier chromosome, and (iii) Recovery of recipient parent genome (RPG). Data on GPC (percent grain weight) was recorded for all selected individual plants from BC2F2:3 generation. The dominant marker Xucw108 was used for foreground selection, and heterozygous plants were identified through progeny testing. For RPG recovery, both genotypic and phenotypic selections were used. Introgression of high GPC gene into recipient background without yield loss was completed in 5 years, starting from F1 (2009-10) and completing it in BC2F5 (2013-2014). Ten selected single plants from BC2F3:4 had comparable yield with 26% higher GPC than the recurrent parent HUW 234. For the other parent HUW 468, eight selected plants had comparable yield with 34% higher GPC. Multi-row progenies (BC2F4 and BC2F5) of each selected plant were evaluated for yield traits with donor and recipient parents during 2012-2013 and 2013-2014. Two lines with significantly higher GPC were identified in each of the crosses with no yield penalty. The study reinforced the belief that marker assisted selection (MAS) in combination with phenotypic selection could be a useful strategy for the development of wheat genotypes with high GPC without sacrificing grain yield.
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