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Sehgal, D.

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Sehgal
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D.
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Sehgal, D.
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0000-0002-4141-1784

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2015 - 20205
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Keyword: Genotyping by Sequencing × Item Type: Publication ×

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Now showing 1 - 5 of 5
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    Genome‐wide association mapping and comparative genomics identifies genomic regions governing grain nutritional traits in finger millet (Eleusine coracana L. Gaertn.)
    (Wiley, 2020) Puranik, S.; Sahu, P.P.; Beynon, S.; Srivastava, R.K.; Sehgal, D.; Ojulong, H.; Yadav, R.S.
    Publication
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    Incorporating genome-wide association mapping results into genomic prediction models for grain yield and yield stability in CIMMYT spring bread wheat
    (Frontiers, 2020) Sehgal, D.; Rosyara, U.; Mondal, S.; Singh, R.P.; Poland, J.; Dreisigacker, S.
    Publication
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    Haplotype-based, genome-wide association study reveals stable genomic regions for grain yield in CIMMYT spring bread wheat
    (Frontiers, 2020) Sehgal, D.; Mondal, S.; Crespo Herrera, L.A.; Velu, G.; Juliana, P.; Huerta-Espino, J.; Shrestha, S.; Poland, J.; Singh, R.P.; Dreisigacker, S.
    Publication
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    Mining centuries old In situ conserved turkish wheat landraces for grain yield and stripe rust resistance genes
    (Frontiers, 2016) Sehgal, D.; Dreisigacker, S.; Belen, S.; Kucukozdemir, U.; Mert, Z.; Ozer, E.; Morgounov, A.
    Wheat landraces in Turkey are an important genetic resource for wheat improvement. An exhaustive 5-year (2009–2014) effort made by the International Winter Wheat Improvement Programme (IWWIP), a cooperative program between the Ministry of Food, Agriculture and Livestock of Turkey, the International Center for Maize and Wheat Improvement (CIMMYT) and the International Center for Agricultural Research in the Dry Areas (ICARDA), led to the collection and documentation of around 2000 landrace populations from 55 provinces throughout Turkey. This study reports the genetic characterization of a subset of bread wheat landraces collected in 2010 from 11 diverse provinces using genotyping-by-sequencing (GBS) technology. The potential of this collection to identify loci determining grain yield and stripe rust resistance via genome-wide association (GWA) analysis was explored. A high genetic diversity (diversity index = 0.260) and a moderate population structure based on highly inherited spike traits was revealed in the panel. The linkage disequilibrium decayed at 10 cM across the whole genome and was slower as compared to other landrace collections. In addition to previously reported QTL, GWA analysis also identified new candidate genomic regions for stripe rust resistance, grain yield, and spike productivity components. New candidate genomic regions reflect the potential of this landrace collection to further increase genetic diversity in elite germplasm.
    Publication
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    A high density GBS map of bread wheat and its application for dissecting complex disease resistance traits
    (BioMed Central, 2015) Huihui Li; Vikram, P.; Singh, R.P.; Kilian, A.; Carling, J.; Jie Song; Burgueño, J.; Bhavani, S.; Huerta-Espino, J.; Payne, T.S.; Sehgal, D.; Wenzl, P.; Singh, S.
    Genotyping-by-sequencing (GBS) is a high-throughput genotyping approach that is starting to be used in several crop species, including bread wheat. Anchoring GBS tags on chromosomes is an important step towards utilizing them for wheat genetic improvement. Here we use genetic linkage mapping to construct a consensus map containing 28644 GBS markers. Results: Three RIL populations, PBW343 × Kingbird, PBW343 × Kenya Swara and PBW343 × Muu, which share a common parent, were used to minimize the impact of potential structural genomic variation on consensus-map quality. The consensus map comprised 3757 unique positions, and the average marker distance was 0.88 cM, obtained by calculating the average distance between two adjacent unique positions. Significant variation of segregation distortion was observed across the three populations. The consensus map was validated by comparing positions of known rust resistance genes, and comparing them to wheat reference genome sequences recently published by the International Wheat Genome Sequencing Consortium, Rye and Ae. tauschii genomes. Three well-characterized rust resistance genes (Sr58/Lr46/Yr29, Sr2/Yr30/Lr27, and Sr57/Lr34/Yr18) and 15 published QTLs for wheat rusts were validated with high resolution. Fifty-two per cent of GBS tags on the consensus map were successfully aligned through BLAST to the right chromosomes on the wheat reference genome sequence. Conclusion: The consensus map should provide a useful basis for analyzing genome-wide variation of complex traits. The identified genes can then be explored as genetic markers to be used in genomic applications in wheat breeding.
    Publication