Person:
Long Mao

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Long Mao
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Long Mao
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0000-0002-3377-4040

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    Origin and evolution of the bread wheat D genome
    (Nature Publishing Group, 2024) Cavalet-Giorsa, E.; Gonzalez-Munoz, A.; Naveenkumar Athiyannan; Holden, S.; Adil Salhi; Gardener, C.; Quiroz-Chávez, J.; Rustamova, S.M.; Elkot, A.F.; Patpour, M.; Awais Rasheed; Long Mao; Lagudah, E.S.; Periyannan, S.; Sharon, A.; Himmelbach, A.; Reif, J.C.; Knauft, M.; Mascher, M.; Stein, N.; Chayut, N.; Ghosh, S.; Perovic, D.; Putra, A.; Perera, A.B.; Chia-Yi Hu; Guotai Yu; Hanin Ibrahim Ahmed; Laquai, K.D.; Rivera, L.F.; Renjie Chen; Yajun Wang; Xin Gao; Liu, S.; Raupp, W.J.; Olson, E.; Jong-Yeol Lee; Chhuneja, P.; Kaur, S.; Peng Zhang; Park, R.F.; Yi Ding; Deng-Cai Liu; Wanlong Li; Nasyrova, F.Y.; Dvorak, J.; Mehrdad Abbasi; Meng Li; Kumar, N.; Meyer, W.B.; Boshoff, W.H.P.; Steffenson, B.J.; Matny, O.; Sharma, P.K.; Tiwari, V.K.; Grewal, S.; Pozniak, C.J.; Harmeet Singh Chawla; Ens, J.; Dunning, L.T.; Kolmer, J.A.; Lazo, G.R.; Xu, S.; Yong Q. Gu; Xianyang Xu; Uauy, C.; Abrouk, M.; Bougouffa, S.; Brar, G.S.; Wulff, B.B.H.; Krattinger, S.G.
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    Population genomic analysis of Aegilops tauschii identifies targets for bread wheat improvement
    (Nature Publishing Group, 2022) Gaurav, K.; Sanu Arora; Silva, P.; Sánchez-Martín, J.; Horsnell, R.; Liangliang Gao; Brar, G.S.; Widrig, V.; Raupp, W.J.; Singh, N.; Shuangye Wu; Kale, S.; Chinoy, C.; Nicholson, P.; Quiroz-Chávez, J.; Simmonds, J.; Hayta, S.; Smedley, M.A.; Harwood, W.; Pearce, S.; Gilbert, D.; Ngonidzashe Kangara; Gardener, C.; Forner-Martínez, M.; Jiaqian Liu; Guotai Yu; Boden, S.A.; Pascucci, A.; Ghosh, S.; Hafeez, A.N.; O’Hara, T.; Waites, J.; Cheema, J.; Steuernagel, B.; Patpour, M.; Justesen, A.F.; Shuyu Liu; Rudd, J.C.; Avni, R.; Sharon, A.; Steiner, B.; Kirana, R.P.; Buerstmayr, H.; Mehrabi, A.A.; Nasyrova, F.Y.; Chayut, N.; Matny, O.; Steffenson, B.; Sandhu, N.; Chhuneja, P.; Lagudah, E.; Elkot, A.F.; Tyrrell, S.; Xingdong Bian; Davey, R.; Simonsen, M.; Schauser, L.; Tiwari, V.K.; Kutcher, H.R.; Hucl, P.J.; Aili Li; Liu Dengcai; Long Mao; Xu, S.; Brown-Guedira, G.; Faris, J.; Dvorak, J.; Ming-Cheng Luo; Krasileva, K.; Lux, T.; Artmeier, S.; Mayer, K.; Uauy, C.; Mascher, M.; Bentley, A.R.; Keller, B.; Poland, J.; Wulff, B.B.H.
    Publication
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    Synthetic hexaploid wheat: yesterday, today, and tomorrow
    (Elsevier, 2018) Aili Li; Liu Dengcai; Wuyun Yang; Kishii, M.; Long Mao
    In recent years, wheat yield per hectare appears to have reached a plateau, leading to concerns for future food security with an increasing world population. Since its invention, synthetic hexaploid wheat (SHW) has been shown to be an effective genetic resource for transferring agronomically important genes from wild relatives to common wheat. It provides new sources for yield potential, drought tolerance, disease resistance, and nutrient-use efficiency when bred conventionally with modern wheat varieties. SHW is becoming more and more important for modern wheat breeding. Here, we review the current status of SHW generation, study, and application, with a particular focus on its contribution to wheat breeding. We also briefly introduce the most recent progress in our understanding of the molecular mechanisms for growth vigor in SHW. Advances in new technologies have made the complete wheat reference genome available, which offers a promising future for the study and applications of SHW in wheat improvement that are essential to meet global food demand.
    Publication
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    An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design
    (Nature Publishing Group, 2016) Yongping Zhao; Congsheng Zhang; Wenwen Liu; Wei Gao; Changlin Liu; Gaoyuan Song; Wen-Xue Li; Long Mao; Beijiu Cheng; Yunbi Xu; Xinhai Li; Chuanxiao Xie
    Precision DNA/gene replacement is a promising genome-editing tool that is highly desirable for molecular engineering and breeding by design. Although the CRISPR/Cas9 system works well as a tool for gene knockout in plants, gene replacement has rarely been reported. Towards this end, we first designed a combinatory dual-sgRNA/Cas9 vector (construct #1) that successfully deleted miRNA gene regions (MIR169a and MIR827a). The deletions were confirmed by PCR and subsequent sequencing, yielding deletion efficiencies of 20% and 24% on MIR169a and MIR827a loci, respectively. We designed a second structure (construct #2) that contains sites homologous to Arabidopsis TERMINAL FLOWER 1 (TFL1) for homology-directed repair (HDR) with regions corresponding to the two sgRNAs on the modified construct #1. The two constructs were co-transformed into Arabidopsis plants to provide both targeted deletion and donor repair for targeted gene replacement by HDR. Four of 500 stably transformed T0 transgenic plants (0.8%) contained replaced fragments. The presence of the expected recombination sites was further confirmed by sequencing. Therefore, we successfully established a gene deletion/replacement system in stably transformed plants that can potentially be utilized to introduce genes of interest for targeted crop improvement.
    Publication
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    Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation
    (Springer Nature, 2013) Jun Wang; Jizeng Jia; Shancen Zhao; Xiuying Kong; Yingrui Li; Guangyao Zhao; Weiming He; Appels, R.; Pfeifer, M.; Yong Tao; Xueyong Zhang; Ruilian Jing; Chi Zhang; Youzhi Ma; Lifeng Gao; Chuan Gao; Spannagl, M.; Mayer, K.; Dong Li; Shengkai Pan; Fengya Zheng; Qun Hu; Xianchun Xia; Jianwen Li; Qinsi Liang; Jie Chen; Wicker, T.; Caiyun Gou,; Hanhui Kuang; Genyun He; Yadan Luo; Keller, B.; Qiuju Xia,; Peng Lu; Junyi Wang; Hongfeng Zou; Rongzhi Zhang; Junyang Xu; Jinlong Gao; Middleton, C.P.; Zhiwu Quan; Guangming Liu; Jian Wang; Huanming Yang; Xu Liu; He Zhonghu; Long Mao
    About 8,000 years ago in the Fertile Crescent, a spontaneous hybridization of the wild diploid grass Aegilops tauschii (2n = 14; DD) with the cultivated tetraploid wheat Triticum turgidum (2n = 4x = 28; AABB) resulted in hexaploid wheat (T. aestivum; 2n = 6x = 42; AABBDD)1, 2. Wheat has since become a primary staple crop worldwide as a result of its enhanced adaptability to a wide range of climates and improved grain quality for the production of baker?s flour2. Here we describe sequencing the Ae. tauschii genome and obtaining a roughly 90-fold depth of short reads from libraries with various insert sizes, to gain a better understanding of this genetically complex plant. The assembled scaffolds represented 83.4% of the genome, of which 65.9% comprised transposable elements. We generated comprehensive RNA-Seq data and used it to identify 43,150 protein-coding genes, of which 30,697 (71.1%) were uniquely anchored to chromosomes with an integrated high-density genetic map. Whole-genome analysis revealed gene family expansion in Ae. tauschii of agronomically relevant gene families that were associated with disease resistance, abiotic stress tolerance and grain quality. This draft genome sequence provides insight into the environmental adaptation of bread wheat and can aid in defining the large and complicated genomes of wheat species.
    Publication