Person: Tadele Tefera
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Tadele Tefera
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Tadele Tefera
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0000-0001-6373-0842 18 results
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- Performance of Bt maize event MON810 in controlling maize stem borers Chilo partellus and Busseola fusca in Uganda(Elsevier, 2022) Otim, M.; Alibu. S.; Asea, G.; Abalo, G.; Sserumaga, J.P.; Adumo, S.; Alupo, J.; Ochen, S.; Tadele Tefera; Bruce, A.Y.; Beyene, Y.; Meisel, B.; Tende, R.; Nang’ayo, F.; Baguma, Y.; Mugo, S.N.; Oikeh, S.O.
Publication - Phenotypic and genotypic variation in tropical maize inbred lines for resistance to the maize weevil and larger grain borer(Transstellar Journal Publications, 2012) Mwololo, J.; Okori, P.; Mugo, S.N.; Tadele Tefera; Beyene, Y.; Otim, M.; Munyiri, S.W.
Publication - Combined effect of hermetic bag and insect resistant variety for the control of larger grain borer and maize weevil in stored maize(International Journal of Science and Research, 2015) Likhayo, P.; Tadele Tefera; Mugo, S.N.; Mueke, J.
Publication - Antibiosis mechanism of resistance to Larger Grain Borer, Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) in maize(Asian Network for Scientific Information, 2014) Nhamucho, E.; Mugo, S.N.; Kinyua, M.G.; Gohole, L.; Tadele Tefera; Mulima, E.P.
Publication - Resistance of Bt-maize (MON810) against the stem borers Busseola fusca (Fuller) and Chilo partellus (Swinhoe) and its yield performance in Kenya(Elsevier, 2016) Tadele Tefera; Mugo, S.N.; Mwimali, M.; Bruce, A.Y.; Tende, R.; Beyene, Y.; Gichuki, S.T.; Oikeh, S.O.; Nang’ayo, F.; Okeno, J.; Njeru, E.; Pillay, K.; Meisel, B.; Prasanna, B.M.A study was conducted to assess the performance of maize hybrids with Bt event MON810 (Bt-hybrids) against the maize stem borer Busseola fusca (Fuller) in a biosafety greenhouse (BGH) and against the spotted stem borer Chilo partellus (Swinhoe) under confined field trials (CFT) in Kenya for three seasons during 2013e2014. The study comprised 14 non-commercialized hybrids (seven pairs of near-isogenic Bt and non-Bt hybrids) and four non-Bt commercial hybrids. Each plant was artificially infested twice with 10 first instar larvae. In CFT, plants were infested with C. partellus 14 and 24 days after planting; in BGH, plants were infested with B. fusca 21 and 31 days after planting. In CFT, the seven Bt hybrids significantly differed from their non-Bt counterparts for leaf damage, number of exit holes, percent tunnel length, and grain yield. When averaged over three seasons, Bt-hybrids gave the highest grain yield (9.7 t ha1), followed by non-Bt hybrids (6.9 t ha1) and commercial checks (6 t ha1). Bt-hybrids had the least number of exit holes and percent tunnel length in all the seasons as compared to the non-Bt hybrids and commercial checks. In BGH trials, Bt-hybrids consistently suffered less leaf damage than their non-Bt near isolines. The study demonstrated that MON810 was effective in controlling B. fusca and C. partellus. Bt-maize, therefore, has great potential to reduce the risk of maize grain losses in Africa due to stem borers, and will enable the smallholder farmers to produce high-quality grain with increased yield, reduced insecticide inputs, and improved food security.
Publication - Responses of tropical maize landraces to damage by Chilo partellus stem borer(Academic Journals, 2013) Munyiri, S.W.; Mugo, S.N.; Otim, M.; Tadele Tefera; Beyene, Y.; Mwololo, J.; Okori, P.The potential to manage insect pests using host-plant resistance exists, but has not been exploited adequately. The objective of this study was to determine the resistance of 75 tropical maize landraces through artificial infestation with Chilo partellusSwinhoe. The trial was laid in alpha-lattice design and each seedling was infested with five neonates three weeks after planting, over two seasons in 2009 and 2010. The number of exit holes, tunnel length, ear diameter, ear length, plant height, stem diameter, stem lodging and grain yield were measured and a selection index computed. GUAT 1050 was the most resistant with an index of 0.56, while BRAZ 2179 was the most susceptible with an index of 1.66. Ear characteristics were negatively correlated with damage parameters. The principal component biplot suggested that exit holes, cumulative tunnel length, leaf damage, cob diameter, stem lodging, selection index, ear and plant height contributed 71.2% of the variation in resistance. The mean number of exit holes and tunnel length for resistant landraces and resistant hybrid checks were similar; at 5.5 and 2.48 cm, respectively. The identified resistant landraces (GUAT 1050, GUAT 280, GUAT 1093, GUAT 1082, GUAT 1014, CHIS 114, and GUAN 34) could be used to develop C. partellus stem borer-resistant maize genotypes.
Publication - Quality of field collected and laboratory reared Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) for screening maize genotypes(Academic Journals, 2013) Mutisya, D.; Tadele Tefera; Mugo, S.N.The quality of laboratory reared stem borer species for screening of maize varieties is usually questioned by end user cereal breeders. A quality check study was performed in a screen house at KARI-Katumani to evaluate the quality of eight-year old laboratory reared stem borer, Chilo partellus (Swinhoe). The evaluation was aimed at finding out the performance of the laboratory borers subjected to six-month interval of gene infusion in comparison with wild F1 generation of the same species collected from the field. One hundred (100) maize seedlings were grown on plastic pots of 5 by 5 cm and of 12 cm-height. The maize seedlings were infested with five first instar larvae on eight plants replicated four times for each borer ecotype. The wild ecotypes were collected from two different localities for comparison with eight-year old laboratory reared borers. Foliar damage, tunnel length on the maize stems and the recovered number of C. partellus larvae from the maize plants were used as the parameters for quality measure of the borer ecotypes. The laboratory-reared stem borer species had been subjected to frequent six-month gene-infusion interval from the wild. The results indicated feedvoracity drop of 3.8 and 21.5% for stem and foliar damage on the laboratory borer ecotype. The study established the need for continuous gene infusion to maintain high quality maize stem borer species as test organisms.
Publication - Sources of resistance to the maize weevil Sitophilus Zeamais in tropical maize(Canadian Center of Science and Education, 2012) Mwololo, J.; Mugo, S.N.; Okori, P.; Tadele Tefera; Otim, M.; Munyiri, S.W.The maize weevil, Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae), is among the major storage pests that enhance food insecurity among maize farmers. New sources of resistance to the maize weevil are critical in a successful breeding program to address grain damage by postharvest pests. The objective of the study was to evaluate resistance in maize genotypes to the maize weevil, and consequently their value for use in breeding programs. A total of 175 genotypes, including hybrids, landraces, open-pollinated varieties and checks, were tested for resistance to the maize weevil. The percentage grain damage, weight loss, flour weight and weight of damaged and undamaged grains were measured. Significant differences (P <0.001) were observed among the genotypes for all the traits evaluated. The distribution of the genotypes among the different categories of resistance was an indication of the existence of genetic variation. The most resistant genotypes were CKPH08003 and BRAZ 2451 while the most susceptible were PH 3254 and BRAZ 4, among the hybrids and landraces respectively. Genotypes that were superior to the resistant checks were identified. The percentage weight loss and flour weight were identified as the most important insect-resistance traits for discriminating genotypes as evident from the canonical discriminant analysis. Correlation coefficients among the traits evaluated were highly significant. The resistant hybrids identified can be recommended for release and adoption by farmers, whereas the resistant landraces can act as sources of resistance for use in breeding programs.
Publication - Testcross performance of doubled haploid maize lines derived from tropical adapted backcross populations(Consiglio per la Ricerca e la sperimentazione in Agricoltura, Unità di Ricerca per la Maiscoltura, 2011) Beyene, Y.; Mugo, S.N.; Pillay, K.; Tadele Tefera; Ajanga, S.; Njoka, S.; Karaya, H.; Gakunga, J.Doubled haploid (DH) lines produced by in vivo induction of maternal haploids are routinely used in maize breeding. The present study was carried out to assess the performance of 75 doubled haploid maize testcrosses and six checks tested across four locations in Kenya for grain yield, agronomic traits and reaction to major leaf diseases. The 75 DH lines were derived from the backcross (BC1) plants of two CIMMYT bi-parental crosses. Significant location, genotype and genotype x location effects were observed for grain yield and anthesis-silking interval (ASI). Genotypes were significantly different for reaction to leaf blight and gray leaf spot. Location explained 69% of the total phenotypic variance while both genotype and genotype by environment interaction effects contributed 4% each. Fifteen DH testcross hybrids yielded better than the best commercial check, WH505 (5.1 t/ha). The best DH testcross hybrid (CKDHH0223) averaged over the four locations yielded 29.5% higher than WH505. These results indicate that maize testcrosses developed from DH lines produced as high a grain yield and as acceptable agronomic traits as the commercial hybrids developed through conventional pedigree methods. The DH lines identified in the study may be useful for improving yield and disease resistance in maize breeding programs in eastern and southern Africa.
Publication - Yield stability of stem borer resistant maize hybrids evaluated in regional trials in east Africa(Academic Journals, 2012) Beyene, Y.; Mugo, S.N.; Tadele Tefera; Gethi, J.; Gakunga, J.; Ajanga, S.; Karaya, H.; Musila, R.N.; Muasya, W.; Tende, R.; Njoka, S.Twenty-seven stem borer-resistant maize hybrids and three checks were evaluated in 14 locations in Kenya and Ethiopia to study the genotype x environment interaction (GEI) and yield stability. An analysis of variance was conducted for grain yield, number of days to silking, plant height, ear height and grain moisture content, and reaction to turcicum leaf blight, gray leaf spot, maize streak virus diseases and common rust. The yield stability and adaptation pattern of genotypes were examined with genotype plus genotype x environment (GGE) interaction biplot. Variations due to location, genotype and GEI effects were highly significant for all traits. Location variance among the hybrids was the most important source of variation for all traits, accounting for 58 to 90% of the total variance. The genotypic variance was higher than the GEI variance for turcicum leaf blight, plant height and silking date. The GEI variance was higher than the genotypic variance for grain yield, ear height, gray leaf spot, common rust and grain moisture content. The GGE biplot showed that 50% of the entries had positive PC1 scores suggesting above average performance, and 50% of them had negative PC1 scores indicating below average performance. Based on the mean grain yield and stability parameters, hybrid CKIR07003 (5.5 t/ha), CKIR07004 (5.5 t/ha) and CKIR07005 (5.6 t /ha) were identified as high-yielding and stable genotypes, and could be nominated for national performance trials for commercial release in various countries.
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