Person: González Pérez, L.
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González Pérez
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L.
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González Pérez, L.
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0000-0002-5840-08033 results
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- High throughput field phenotyping for plant height using UAV-based RGB imagery in wheat breeding lines: feasibility and validation(Frontiers, 2021) Volpato, L.; Pinto Espinosa, F.; González Pérez, L.; Thompson, I.G.; Borem, A.; Reynolds, M.P.; Gerard, B.; Molero, G.; Rodrigues, F.
Publication - Estimation of physiological genomic estimated breeding values (PGEBV) combining full hyperspectral and marker data across environments for grain yield under combined heat and drought stress in tropical maize (Zea mays L.)(Public Library of Science, 2019) Trachsel, S.; Dhliwayo, T.; González Pérez, L.; Mendoza Lugo, J.A.; Trachsel, M.High throughput phenotyping technologies are lagging behind modern marker technology impairing the use of secondary traits to increase genetic gains in plant breeding. We aimed to assess whether the combined use of hyperspectral data with modern marker technology could be used to improve across location pre-harvest yield predictions using different statistical models. A maize bi-parental doubled haploid (DH) population derived from F1, which consisted of 97 lines was evaluated in testcross combination under heat stress as well as combined heat and drought stress during the 2014 and 2016 summer season in Ciudad Obregon, Sonora, Mexico (27°20” N, 109°54” W, 38 m asl). Full hyperspectral data, indicative of crop physiological processes at the canopy level, was repeatedly measured throughout the grain filling period and related to grain yield. Partial least squares regression (PLSR), random forest (RF), ridge regression (RR) and Bayesian ridge regression (BayesB) were used to assess prediction accuracies on grain yield within (two-fold cross-validation) and across environments (leave-one-environment-out-cross-validation) using molecular markers (M), hyperspectral data (H) and the combination of both (HM). Highest prediction accuracy for grain yield averaged across within and across location predictions (rGP) were obtained for BayesB followed by RR, RF and PLSR. The combined use of hyperspectral and molecular marker data as input factor on average had higher predictions for grain yield than hyperspectral data or molecular marker data alone. The highest prediction accuracy for grain yield across environments was measured for BayesB when molecular marker data and hyperspectral data were used as input factors, while the highest within environment prediction was obtained when BayesB was used in combination with hyperspectral data. It is discussed how the combined use of hyperspectral data with molecular marker technology could be used to introduce physiological genomic estimated breeding values (PGEBV) as a pre-harvest decision support tool to select genetically superior lines.
Publication - Hyperspectral reflectance-derived relationship matrices for genomic prediction of grain yield in wheat(Genetics Society of America, 2019) Krause, M.; González Pérez, L.; Crossa, J.; Pérez-Rodríguez, P.; Montesinos-Lopez, O.A.; Singh, R.P.; Dreisigacker, S.; Poland, J.; Rutkoski, J.; Sorrells, M.E.; Gore, M.A.; Mondal, S.Hyperspectral reflectance phenotyping and genomic selection are two emerging technologies that have the potential to increase plant breeding efficiency by improving prediction accuracy for grain yield. Hyperspectral cameras quantify canopy reflectance across a wide range of wavelengths that are associated with numerous biophysical and biochemical processes in plants. Genomic selection models utilize genome-wide marker or pedigree information to predict the genetic values of breeding lines. In this study, we propose a multi-kernel GBLUP approach to genomic selection that uses genomic marker-, pedigree-, and hyperspectral reflectance-derived relationship matrices to model the genetic main effects and genotype × environment (G × E) interactions across environments within a bread wheat (Triticum aestivum L.) breeding program. We utilized an airplane equipped with a hyperspectral camera to phenotype five differentially managed treatments of the yield trials conducted by the Bread Wheat Improvement Program of the International Maize and Wheat Improvement Center (CIMMYT) at Ciudad Obregón, México over four breeding cycles. We observed that single-kernel models using hyperspectral reflectance-derived relationship matrices performed similarly or superior to marker- and pedigree-based genomic selection models when predicting within and across environments. Multi-kernel models combining marker/pedigree information with hyperspectral reflectance phentoypes had the highest prediction accuracies; however, improvements in accuracy over marker- and pedigree-based models were marginal when correcting for days to heading. Our results demonstrate the potential of using hyperspectral imaging to predict grain yield within a multi-environment context and also support further studies on the integration of hyperspectral reflectance phenotyping into breeding programs.
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