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Genying Li

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Genying Li
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Genying Li

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Now showing 1 - 3 of 3
  • Thumbnail Image
    Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
    (Institute of Crop Sciences, 2007) Genying Li; Xianchun Xia; He Zhonghu; Sun, Qi-Xin
    Publication
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    Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat
    (BioMed Central, 2012) Xiaofei Zhang; Hui Jin; Zhang, Y.; Dongcheng Liu; Genying Li; Xianchun Xia; He Zhonghu; Zhang Aimin
    Low-molecular-weight glutenin subunits (LMW-GS) strongly influence the bread-making quality of bread wheat. These proteins are encoded by a multi-gene family located at the Glu-A3, Glu-B3 and Glu-D3 loci on the short arms of homoeologous group 1 chromosomes, and show high allelic variation. To characterize the genetic and protein compositions of LMWGS alleles, we investigated 16 Aroona near-isogenic lines (NILs) using SDS-PAGE, 2DPAGE and the LMW-GS gene marker system. Moreover, the composition of glutenin macropolymers, dough properties and pan bread quality parameters were determined for functional analysis of LMW-GS alleles in the NILs. Results Using the LMW-GS gene marker system, 14?20 LMW-GS genes were identified in individual NILs. At the Glu-A3 locus, two m-type and 2?4 i-type genes were identified and their allelic variants showed high polymorphisms in length and nucleotide sequences. The Glu-A3d allele possessed three active genes, the highest number among Glu-A3 alleles. At the Glu-B3 locus, 2?3 m-type and 1?3 s-type genes were identified from individual NILs. Based on the different compositions of s-type genes, Glu-B3 alleles were divided into two groups, one containing Glu-B3a, B3b, B3f and B3g, and the other comprising Glu-B3c, B3d, B3h and B3i. Eight conserved genes were identified among Glu-D3 alleles, except for Glu-D3f. The protein products of the unique active genes in each NIL were detected using protein electrophoresis. Among Glu-3 alleles, the Glu-A3e genotype without i-type LMW-GS performed worst in almost all quality properties. Glu-B3b, B3g and B3i showed better quality parameters than the other Glu-B3 alleles, whereas the Glu-B3c allele containing s-type genes with low expression levels had an inferior effect on bread-making quality. Due to the conserved genes at Glu-D3 locus, Glu-D3 alleles showed no significant differences in effects on all quality parameters. Conclusions This work provided new insights into the composition and function of 18 LMW-GS alleles in bread wheat. The variation of i-type genes mainly contributed to the high diversity of Glu-A3 alleles, and the differences among Glu-B3 alleles were mainly derived from the high polymorphism of s-type genes. Among LMW-GS alleles, Glu-A3e and Glu-B3c represented inferior alleles for bread-making quality, whereas Glu-A3d, Glu-B3b, Glu-B3g and Glu-B3i were correlated with superior bread-making quality. Glu-D3 alleles played minor roles in determining quality variation in bread wheat. Thus, LMW-GS alleles not only affect dough extensibility but greatly contribute to the dough resistance, glutenin macro-polymers and bread quality.
    Publication
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    Distribution of grain hardness and puroindoline alleles in landraces, historical and current wheats in Shandong province
    (Institute of Crop Sciences, 2007) Genying Li; Xianchun Xia; He Zhonghu; Sun, Qi-Xin; Huang Cheng-Yan
    Studies on the grain hardness and puroindolines alleles in wheat cultivars released in different historical periods, are helpful for breeding wheat cultivar with optimal endosperm texture. In the present study, 523 accessions from Shandong Province including 431 landraces, 63 historical cultivars and 29 current cultivars were used to evaluate the SKCS hardness and distribution of puroindoline alleles (Pins). Distribution of grain hardness differed in landraces, historical cultivars and current wheats, with 75.6%, 20.4%, and 3.9% of hard texture, and 20.4%, 19.0%, and 13.8% of mixed wheats, and 3.9%, 68.3%, and 58.6% of soft grains, respectively. Six genotypes of Pina and Pinb were present in landraces, in which Pina-D1a/Pinb-D1p and Pina-D1b/Pinb-D1b were the dominant genotypes, accounting for 38.0% and 59.6% of hard wheat, respectively. Compared with landraces, the polymorphism of Pina and Pinb was decreased in historical cultivars. Pina-D1b/Pinb-D1a, Pina-D1a/Pinb-D1b, and Pina-D1a/Pinb-D1p accounted for 37.5%, 37.5%, and 25.0% of hard wheat, respectively, whereas, Pina-D1a/Pinb-D1b was the only genotype presented in hard genotype of current cultivars surveyed. A novel Pinb allele with double mutations at the positions of 96th (C to A) and 265th (deletion of A) was found in three landraces, and was designated as Pinb-D1aa.
    Publication