Person: Kishii, M.
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Kishii
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Kishii, M.
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0000-0002-3646-38544 results
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- Harnessing translational research in wheat for climate resilience(Oxford University Press, 2021) Reynolds, M.P.; Lewis, J.; Ammar, K.; Basnet, B.R.; Crespo Herrera, L.A.; Crossa, J.; Dhugga, K.; Dreisigacker, S.; Juliana, P.; Karwat, H.; Kishii, M.; Krause, M.; Langridge, P.; Lashkari, A.; Mondal, S.; Payne, T.S.; Pequeno, D.N.L.; Pinto Espinosa, F.; Sansaloni, C.; Schulthess, U.; Singh, R.P.; Sonder, K.; Sukumaran, S.; Wei Xiong; Braun, H.J.
Publication - Genetic contribution of synthetic hexaploid wheat to CIMMYT’s spring bread wheat breeding germplasm(Nature Publishing Group, 2019) Rosyara, U.; Kishii, M.; Payne, T.S.; Sansaloni, C.; Singh, R.P.; Braun, H.J.; Dreisigacker, S.Synthetic hexaploid (SH) wheat (AABBD’D’) is developed by artificially generating a fertile hybrid between tetraploid durum wheat (Triticum turgidum, AABB) and diploid wild goat grass (Aegilops tauschii, D’D’). Over three decades, the International Maize and Wheat Improvement Center (CIMMYT) has developed and utilized SH wheat to bridge gene transfer from Ae. tauschii and durum wheat to hexaploid bread wheat. This is a unique example of success utilizing wild relatives in mainstream breeding at large scale worldwide. Our study aimed to determine the genetic contribution of SH wheat to CIMMYT’s global spring bread wheat breeding program. We estimated the theoretical and empirical contribution of D’ to synthetic derivative lines using the ancestral pedigree and marker information using over 1,600 advanced lines and their parents. The average marker-estimated D’ contribution was 17.5% with difference in genome segments suggesting application of differential selection pressure. The pedigree-based contribution was correlated with marker-based estimates without providing chromosome segment specific variation. Results from international yield trials showed that 20% of the lines were synthetic derived with an average D’ contribution of 15.6%. Our results underline the importance of SH wheat in maintaining and enhancing genetic diversity and genetic gain over years and is important for development of a more targeted introgression strategy. The study provides retrospective view into development and utilization of SH in the CIMMYT Global Wheat Program.
Publication - An update of recent use of Aegilops species in wheat breeding(Frontiers, 2019) Kishii, M.Aegilops species have significantly contributed to wheat breeding despite the difficulties involved in the handling of wild species, such as crossability and incompatibility. A number of biotic resistance genes have been identified and incorporated into wheat varieties from Aegilops species, and this genus is also contributing toward improvement of complex traits such as yield and abiotic tolerance for drought and heat. The D genome diploid species of Aegilops tauschii has been utilized most often in wheat breeding programs. Other Aegilops species are more difficult to utilize in the breeding because of lower meiotic recombination frequencies; generally they can be utilized only after extensive and time-consuming procedures in the form of translocation/introgression lines. After the emergence of Ug99 stem rust and wheat blast threats, Aegilops species gathered more attention as a form of new resistance sources. This article aims to update recent progress on Aegilops species, as well as to cover new topics around their use in wheat breeding.
Publication - Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines(Springer Nature, 2018) Edet, O.U.; Gorafi, Y.; Seong-woo Cho; Kishii, M.; Tsujimoto, H.Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530L. mollis SNP markers and the chromosome-specifc SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort.
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